Atypical location of double-strand origin of replication (nic site) on the plasmid pGA1 fromCorynebacterium glutamicum
- 76 Downloads
The double-strand origin of replication (dso) of the rolling-circle-replicating (RC) plasmid pGA1 fromCorynebacterium glutamicum was analyzed using the runoff DNA synthesis assay. The site- and strand-specific breakage of double-stranded plasmid DNA, representing thenic site ofdso, was localized precisely within the sequence 5′-CTGG»AT-3′ in the distal part of the pGA1rep gene. This location ofdso differs from thedso positions found on other RC plasmids and is in agreement with the classification of the plasmid pGA1 into a new group of RC plasmids.
KeywordsCorynebacterium Glutamicum Bacterial Plasmid Cryptic Plasmid Rolling Circle Replication Nucleotide Resolution
Unable to display preview. Download preview PDF.
- Osborn M., Bron S., Firth N., Holsappel S., Huddleston A., Kiewiet R., Meijer W., Seegers J., Skurray R., Terpstra P., Thomas C.M., Thorsted P., Tietze E., Turner S.L.: The evolution of bacterial plasmids, pp. 301–361 in C.M. Thomas (Ed.):The Horizontal Gene Pool: Bacterial Plasmid and Gene Spread. Harwood Academic Publishers, Amsterdam (The Netherlands) 2000.Google Scholar
- Tauch A., Krieft S., Kalinowski J., Pühler A.: The 51,409-bp R-plasmid pTP10 from the multiresistant clinical isolateCorynebacterium striatum M82B is composed of DNA segments initially identified in soil bacteria and in plant, animal, and human pathogens.Mol. Gen. Genet. 263, 1–11 (2000b).PubMedCrossRefGoogle Scholar