Folia Microbiologica

, Volume 47, Issue 4, pp 307–310 | Cite as

Atypical location of double-strand origin of replication (nic site) on the plasmid pGA1 fromCorynebacterium glutamicum

  • Z. Abrhámová
  • M. Pátek
  • J. Nešvera


The double-strand origin of replication (dso) of the rolling-circle-replicating (RC) plasmid pGA1 fromCorynebacterium glutamicum was analyzed using the runoff DNA synthesis assay. The site- and strand-specific breakage of double-stranded plasmid DNA, representing thenic site ofdso, was localized precisely within the sequence 5′-CTGG»AT-3′ in the distal part of the pGA1rep gene. This location ofdso differs from thedso positions found on other RC plasmids and is in agreement with the classification of the plasmid pGA1 into a new group of RC plasmids.


Corynebacterium Glutamicum Bacterial Plasmid Cryptic Plasmid Rolling Circle Replication Nucleotide Resolution 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2002

Authors and Affiliations

  • Z. Abrhámová
    • 1
  • M. Pátek
    • 1
  • J. Nešvera
    • 1
  1. 1.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPragueCzechia

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