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Folia Microbiologica

, Volume 47, Issue 6, pp 723–726 | Cite as

Detection ofpap-,sfa- andafa-specific DNA sequences inEscherichia coli strains isolated from extraintestinal material

  • E. BogyiováEmail author
  • M. Kmeťová
  • E. Biroš
  • L. Siegfried
Papers

Abstract

P-fimbriae, S-fimbriae and AFA-adhesins are virulence factors responsible for adherence ofEscherichia coli strains to extraintestinal host-cell surface. Detection ofpap-,sfa- andafa-specific sequences performed by PCR revealed 74%pap +, 65%sfa +, and 8.3%afa + strains in a group of 84 extraintestialE. coli isolates. Detection in a group of fecal strains showed 29%pap +, 21%sfa + and 4%afa + strains.pap together withsfa were found as the most frequent combination (56%) among extraintestinal isolates probably due to localization ofpap-andsfa-operons on a common pathogenicity island. The occurrence ofafa-specific sequence among 56 urine strains was 11%, although noafa + strain was detected among 28 gynecological isolates. No strains with detected adhesin operons were found among twenty (24%) extraintestinalE. coli strains.

Keywords

Virulence Factor Coli Strain Polymerase Chain Reaction Detection ofEscherichia Coli Coli Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2002

Authors and Affiliations

  • E. Bogyiová
    • 1
    Email author
  • M. Kmeťová
    • 1
  • E. Biroš
    • 2
  • L. Siegfried
    • 1
  1. 1.Institute of Medical Microbiology, School of MedicineŠafárik UniversityKošiceSlovakia
  2. 2.Laboratory of Genetic Ecotoxicology, Institute of Experimental MedicineAcademy of Sciences of the Czech RepublicPragueCzechia

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