Folia Microbiologica

, Volume 47, Issue 6, pp 723–726 | Cite as

Detection ofpap-,sfa- andafa-specific DNA sequences inEscherichia coli strains isolated from extraintestinal material

  • E. BogyiováEmail author
  • M. Kmeťová
  • E. Biroš
  • L. Siegfried


P-fimbriae, S-fimbriae and AFA-adhesins are virulence factors responsible for adherence ofEscherichia coli strains to extraintestinal host-cell surface. Detection ofpap-,sfa- andafa-specific sequences performed by PCR revealed 74%pap +, 65%sfa +, and 8.3%afa + strains in a group of 84 extraintestialE. coli isolates. Detection in a group of fecal strains showed 29%pap +, 21%sfa + and 4%afa + strains.pap together withsfa were found as the most frequent combination (56%) among extraintestinal isolates probably due to localization ofpap-andsfa-operons on a common pathogenicity island. The occurrence ofafa-specific sequence among 56 urine strains was 11%, although noafa + strain was detected among 28 gynecological isolates. No strains with detected adhesin operons were found among twenty (24%) extraintestinalE. coli strains.


Virulence Factor Coli Strain Polymerase Chain Reaction Detection ofEscherichia Coli Coli Isolate 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2002

Authors and Affiliations

  • E. Bogyiová
    • 1
    Email author
  • M. Kmeťová
    • 1
  • E. Biroš
    • 2
  • L. Siegfried
    • 1
  1. 1.Institute of Medical Microbiology, School of MedicineŠafárik UniversityKošiceSlovakia
  2. 2.Laboratory of Genetic Ecotoxicology, Institute of Experimental MedicineAcademy of Sciences of the Czech RepublicPragueCzechia

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