Folia Microbiologica

, 43:438 | Cite as

Gentamicin resistance in clinical strains ofEnterobacteriaceae associated with reduced gentamicin uptake

  • K. Adwan
  • N. Abu-Hasan


Seven strains ofEnterobacteriaceae resistant to gentamicin obtained as representatives of the predominant resistance profiles in the clinical laboratories ofRafeidia and Al-Watani Hospitals in Nablus (Palestine) were included. Five strains showed a broad aminoglycoside resistance profile but contained no evidence of gentamicin acetylation, adenylation, or phosphorylation. Gentamicin uptake in two tested strains was significantly reduced, compared to that of gentamicin-sensitiveE. coli (MIC, 0.5 μg/mL.) These strains are likely resistant due to a relative reduction of the amount of gentamicin and other aminoglycosides entering the bacterial cell. Two strains showed evidence of adenyltransferase ANT (2")-I activity.


Minimum Inhibitory Concentration Gentamicin Aminoglycoside Amikacin Tobramycin 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1998

Authors and Affiliations

  • K. Adwan
    • 1
  • N. Abu-Hasan
    • 1
  1. 1.Department of Biological SciencesAn-Najah N. UniversityNablusPalestine

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