Gentamicin resistance in clinical strains ofEnterobacteriaceae associated with reduced gentamicin uptake
- 72 Downloads
Seven strains ofEnterobacteriaceae resistant to gentamicin obtained as representatives of the predominant resistance profiles in the clinical laboratories ofRafeidia and Al-Watani Hospitals in Nablus (Palestine) were included. Five strains showed a broad aminoglycoside resistance profile but contained no evidence of gentamicin acetylation, adenylation, or phosphorylation. Gentamicin uptake in two tested strains was significantly reduced, compared to that of gentamicin-sensitiveE. coli (MIC, 0.5 μg/mL.) These strains are likely resistant due to a relative reduction of the amount of gentamicin and other aminoglycosides entering the bacterial cell. Two strains showed evidence of adenyltransferase ANT (2")-I activity.
KeywordsMinimum Inhibitory Concentration Gentamicin Aminoglycoside Amikacin Tobramycin
- Al-Asmar H.: Clinical perspective of aminoglycosides resistance inEnterobacteriaceae andPseudomonas. MSc Thesis. An-Najah N. University, Nablus 1996.Google Scholar
- Bauer A.W., Kirby W.M.M., Sherris J.C., Truck M.: Antibiotic susceptibility testing by a standardized single disk method.J. Gen. Microbiol. 45, 493–496 (1966).Google Scholar
- Ericsson H.M., Sherris J.C.: Antibiotic sensitivity testing: Report of an international collaborative study.Acta Pathol. Microbiol. Scand. B 217, 65–73 (1971).Google Scholar
- Hirai K., Aoyaman H., Suzue S., Irikura T., Iyobe S., Mitsuhashi S.: Isolation and characterization of norfloxacin-resistance mutants ofEscherichia coli K-12.Antimicrob Agents Chemother 20, 248–253 (1986).Google Scholar