Indigenous plasmids in a production line of strains for penicillin G acylase derived fromEscherichia coli W
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Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase, (PGA) derived from the strainEscherichia coli W ATCC 9637. Their size and copy number (CN) inE. coli W were determined (kb; CN); pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). StrainE. coli RE2 harboring these plasmids was used for selection of strains with reduced number of plasmids: the strain RE3 without plasmid pRK1 and the plasmid-less strain cERE3 were isolated. Indigenous plasmids did not code for the resistance determinants against 23 antibiotics and 10 heavy metals.
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- Sambrook J., Fritsch E.F., Maniatis T.:Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 1989.Google Scholar
- Vohradský J., Marešová H., Večerek B., Kyslík P.: Determination of plasmid copy number in continous culture by fluorescence densitometry and image analysis.Methods Mol. Cell. Biol. 5/6, 223–233 (1990).Google Scholar
- Vojtíšek V., Vaněk Z.: Selection and enrichment of active strains of microorganisms and their use for production of immobilized cell biocatalysts, inOverproduction of Microbial Metabolites. Strain Improvement and Process Control Strategies. (Z. Vaněk, Z. Hoštálek, Eds). Butterworth, Boston 1986.Google Scholar