Folia Microbiologica

, Volume 44, Issue 3, pp 263–266 | Cite as

Indigenous plasmids in a production line of strains for penicillin G acylase derived fromEscherichia coli W

  • L. Sobotková
  • J. Grafková
  • V. Štěpánek
  • T. Vacík
  • H. Marešová
  • P. Kyslík
Papers

Abstract

Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase, (PGA) derived from the strainEscherichia coli W ATCC 9637. Their size and copy number (CN) inE. coli W were determined (kb; CN); pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). StrainE. coli RE2 harboring these plasmids was used for selection of strains with reduced number of plasmids: the strain RE3 without plasmid pRK1 and the plasmid-less strain cERE3 were isolated. Indigenous plasmids did not code for the resistance determinants against 23 antibiotics and 10 heavy metals.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1999

Authors and Affiliations

  • L. Sobotková
    • 1
  • J. Grafková
    • 2
  • V. Štěpánek
    • 1
  • T. Vacík
    • 1
  • H. Marešová
    • 1
  • P. Kyslík
    • 1
  1. 1.Laboratory of Enzyme Technology, Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPragueCzech Republic
  2. 2.Faculty of PharmacyCharles UniversityHradec KrálovéCzech Republic

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