Folia Microbiologica

, Volume 46, Issue 6, pp 559–563 | Cite as

Particulate 1,3-β-d-glucan, carboxymethylglucan and sulfoethylglucan—Influence of their oral or intraperitoneal administration on immunological respondence of mice

  • J. Mucksová
  • K. Babíček
  • M. Pospíšil
Immunology Papers


The effect of orally or intraperitoneally administered particulate 1,3-β-d-glucan (PBG), carboxymethylglucan (CMG) or sulfoethylglucan (SEG), obtained from the culture filtrate ofSaccharomyces cerevisiae, on the functions of murine peritoneal adherent cells (PC) (peroxidase activity, nitric oxide synthesis), on relative organ mass and on proliferation of splenocytes was determined. The modulating activities after parenteral and non-parenteral administration of these polysaccharides were compared. Significant enhancement of NO production was observed only afterin vitro cultivation of PC in the presence of lipopolysaccharide (LPS) in groups of mice treated repeatedly orally with CMG, PBG and SEG at a dose of 50 mg/kg body mass. Peroxidase activity increased significantly after repeated oral administration of CMG and PBG at doses 150 and 50 mg/kg, SEG 150 mg/kg body mass. The peroxidase activity and NO synthesis in mice given a single intraperitoneal injection of glucans (15 mg/kg body mass) were slightly higher than those after oral administration. Neither a significant enhancement of relative organ mass nor enhancement of the proliferative response of splenocytes toin vitro added stimuli (LPS, phytohemagglutinin) after repeated oral or single intraperitoneal administration of β-glucans was observed.


Glucan Peritoneal Macrophage Nitric Oxide Synthesis Peritoneal Exudate Cell Tularemia 
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© Folia Microbiologica 2001

Authors and Affiliations

  1. 1.Department of Pathology and Microbiology, BIOPHARMResearch Institute of Biopharmacy and Veterinary DrugsJilové near PragueCzechia
  2. 2.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPragueCzechia

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