Folia Microbiologica

, Volume 47, Issue 3, pp 283–286 | Cite as

Decolorization of synthetic textile dyes by lignin peroxidase ofPhanerochaete chrysosporium

  • P. Verma
  • D. Madamwar
Papers

Abstract

Neem hull waste (containing a high amount of lignin and other phenolic compounds) was used for lignin peroxidase production byPhanerochaete chrysosporum under solid-state fermentation conditions. Maximum decolorization achieved by partially purified lignin peroxidase was 80% for Porocion Brilliant Blue HGR, 83 for Ranocid Fast Blue, 70 for Acid Red 119 and 61 for Navidol Fast Black MSRL. The effects of different concentrations of veratryl alcohol, hydrogen peroxide, enzyme and dye on the efficiency of decolorization have been investigated. Maximum decolorization efficiency was observed at 0.2 and 0.4 mmol/L hydrogen peroxide, 2.5 mmol/L veratryl alcohol and pH 5.0 after a 1-h reaction, using 50 ppm of dyes and 9.96 mkat/L of enzyme.

References

  1. Buckley K.F., Dobson A.D.W.: Extracellular ligninolytic enzyme production and polymeric dye decolorization in immobilized cultures ofChrysosporium lignorum CL 1.Biotechnol. Lett.20, 301–306 (1998).CrossRefGoogle Scholar
  2. Darah I., Ibrahim C.O.: Laboratory-scale production of lignin-degrading enzymes by free and entrapped cells ofPhanerochaete chrysosporium in a tubular air-lift bioreactor.Folia Microbiol.43, 161–168 (1998).Google Scholar
  3. Das N., Chakraborty T.K., Mukherjee M.: Purification and characterization of laccase 1 fromPleurotus florida.Folia Microbiol.45, 447–451 (2000).CrossRefGoogle Scholar
  4. Gabriel J., Shah V., Nesměrák K., Baldrian P., Nerud F.: Degradation of polycyclic aromatic hydrocarbons by the copper(II) hydrogen peroxide system.Folia Microbiol.45, 573–576 (2000).Google Scholar
  5. Hattaka A.: Lignin modifying enzymes from selected white rot fungi: production and role in lignin degradation.FEMS Microbiol. Rev.13, 125–135 (1994).CrossRefGoogle Scholar
  6. Kahraman S., Yešilada O. Effect of spent cotton stalks on color removal and chemical oxygen demand lowering in olive oil mill wastewater by white rot fungi.Folia Microbiol.44, 673–676 (1999).Google Scholar
  7. Kahraman S., Yešilada O.: Industrial and agricultural wastes as substrates for laccase production by white-rot fungi.Folia Microbiol.45, 133–136 (2000).CrossRefGoogle Scholar
  8. Katić M., Frantar J., Grgić I., Podgornik H., Perdih A.: Polyoxyethylene stimulates lignin peroxidase production inPhanerochaete chrysosporium.Folia Microbiol.43, 631–634 (1998).Google Scholar
  9. Kirk T.K., Farrel R.L.: Enzymatic combustion: the microbial degradation of lignin.Ann. Rev. Microbiol.41, 464–505 (1987).CrossRefGoogle Scholar
  10. Knapp J.S., Newby P.S., Reece L.P.: Decolorization of dyes by wood-rotting fungi.Enzyme Microb. Technol.17, 664–668 (1994).CrossRefGoogle Scholar
  11. Kotterman M.J.J., Rietberg H.J., Hage A., Field J.A.: Polycyclic aromatic hydrocarbon oxidation by the white-rot fungusBjerkandera sp. strain BOS55 in the presence of nonionic surfactants.Biotechnol. Bioeng.57, 220–227 (1997).CrossRefGoogle Scholar
  12. Krčmar P., Ulrich R.: Degradation of polychlorinated biphenyl mixtures by the lignin-degrading fungusPhanerochaete chrysosporium.Folia Microbiol.43, 79–84 (1998).CrossRefGoogle Scholar
  13. Martens R., Zadrazil F.: Screening of white-rot fungi for their ability to mineralize polycyclic aromatic hydrocarbons in soil.Folia Microbiol.43, 97–103 (1998).Google Scholar
  14. Mehna A., Bajpai P., Bajpai P.K.: Studies on decolorization of effluent from a small pulp mill utilizing agriresidues withTrametes versicolor.Enzyme Microb. Technol.17, 18–22 (1993).CrossRefGoogle Scholar
  15. Sam M., Yeşilada O.: Decolorization of Orange II dye by white-rot fungi.Folia Microbiol.46, 143–146 (2001).Google Scholar
  16. Sani R.K., Azmi W., Banerjee U.C.: Comparison of static and shake culture in the decolorization of textile dyes and dye effluents byPhanerochaete chrysosporium.Folia Microbiol.43, 85–88 (1998).Google Scholar
  17. Shah V., Garg N., Madamwar D.: Exopolysaccharides production by a marine cyanobacteriumCyanothece sp. and its application in dye removal by its gelation phenomenon.Appl. Biochem. Biotechnol.82, 81–90 (1999).CrossRefGoogle Scholar
  18. Tien M., Krik T.K.: Lignin peroxidase ofPhanerochaete chrysosporium.Meth. Enzymol.161, 238–243 (1988).CrossRefGoogle Scholar
  19. Ünyayar S., Ünal E., Ünyayar A.: Relationship between production of 3-indoleacetic acid and peroxidase-laccase activities depending on the culture periods inFunalia trogii (Trametes trogii).Folia Microbiol.46, 123–126 (2001).Google Scholar
  20. Young L., Yu J.: Ligninase-catalyzed decolorization of synthetic dyes.Water Sci. Technol.31, 1187–1197 (1997).Google Scholar
  21. Zheng Z., Levin R.E., Pinkam J.L., Shetty K.: Decolorization of polymeric dyes by aPenicillium isolate.Proc. Biochem.34, 31–37 (1999).CrossRefGoogle Scholar

Copyright information

© Folia Microbiologica 2002

Authors and Affiliations

  • P. Verma
    • 1
  • D. Madamwar
    • 1
  1. 1.Post-Graduate Department of BiosciencesSardar Patel UniversityGujaratIndia

Personalised recommendations