Molecular and Chemical Neuropathology

, Volume 23, Issue 2–3, pp 103–114

Concomitant cortical expression of TNF-α and IL-1β mRNAs follows early response gene expression in transient focal ischemia

  • Xinkang Wang
  • Tian-Li Yue
  • Frank C. Barone
  • Raymond F. White
  • Robert C. Gagnon
  • Giora Z. Feuerstein


The expression of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) mRNAs was significantly increased in the rat ischemic cortex following temporary occlusion of the middle cerebral artery (TMCAO) with reperfusion. Northern blot analysis demonstrated that the induction of TNF-α and IL-1β mRNAs occurred as early as 1 h after reperfusion, exhibiting a 4.6-fold increase (p<0.05,n=4) and 6.8-fold increase (p<0.05,n=4) in the ischemic cortex over control, respectively. TNF-α mRNA reached its peak at 3 h (8.0-fold,p<0.05), whereas IL-1β mRNA reached its peak at 6 h (29.5-fold,p<0.05). Both cytokine mRNA levels remained elevated for up to 2 d after reperfusion. In contrast to the time course of these cytokine mRNAs, c-fos and zif268 mRNAs, two early response genes, displayed a greater and earlier time-response profile. The early induction of c-fos and zif268 mRNAs in temporary brain ischemia with reperfusion suggests their roles in transcriptional regulation. The later concomitant expression of TNF-α and IL-1β suggests that these cytokines play an important role in the inflammatory response associated with focal ischemia.

Index Entries

TNF-α IL-1β transient focal brain ischemia stroke inflammation 


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Copyright information

© Humana Press Inc 1994

Authors and Affiliations

  • Xinkang Wang
    • 1
  • Tian-Li Yue
    • 1
  • Frank C. Barone
    • 1
  • Raymond F. White
    • 1
  • Robert C. Gagnon
    • 2
  • Giora Z. Feuerstein
    • 1
  1. 1.Department of Cardiovascular PharmacologySmithKline Beecham PharmaceuticalsKing of Prussia
  2. 2.Statistical SciencesSmithKline Beecham PharmaceuticalsKing of Prussia

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