Folia Geobotanica

, Volume 31, Issue 3, pp 315–321

Determination of the ploidy level in the genusHieracium subgenusPilosella (Hill) S.F. Gray by flow cytometric DNA analysis

  • Siegfried Bräutigam
  • Edeltraut Bräutigam
Apomixis and Taxonomy Proceedings of the Symposium held in Pruhonice, Czech Republic 1995; edited by A.J. Richards, J. Kirschner, J. Stepanek & K. Marhold

DOI: 10.1007/BF02815376

Cite this article as:
Bräutigam, S. & Bräutigam, E. Folia Geobot (1996) 31: 315. doi:10.1007/BF02815376

Abstract

Ploidy levels of 8 taxa of the genusHieracium subgenusPilosella collected from the same locality were determined by means of flow cytometric estimation of relative nuclear DNA content. The DNA anaysis of isolated nuclei stained with propidium iodide was performed with a flow cytometer equipped with an argon-ion laser. The diploid speciesH. lactucella (2n=18) was used as internal standard. As a rule 20 individuals per taxon were analysed. The flow cytometric analysis proved to be a rapid, accurate and sensitive method for screening of ploidy levels. Considering the known cytological data, the following ploidy levels were determined:H. aurantiacum 4x,H. pilosella 4x,H. piloselloides 4x,H. bauhini 5x,H. caespitosum 5x,H. stoloniflorum 4x,H. brachiatum 4x and 6x,H. leptophyton 7x.H. piloselloides, H. bauhini andH. caespitosum yield relative DNA contents which are the exact multiples of the haploid DNA content ofH. lactucella. In the case ofH. pilosella the haploid DNA content is lower than that predicted from the diploid, namely 0.43 instead of the expected 0.5. The haploid 1x DNA contents of bothH. brachiatum andH. leptophyton range between the value found forH. pilosella and those for the other investigated species, confirming their hybrid origin.

Keywords

Apomixis Asteraceae Chromosome number Compositae Hybrid origin Nuclear DNA content 

Copyright information

© Institute of Botany 1996

Authors and Affiliations

  • Siegfried Bräutigam
    • 1
  • Edeltraut Bräutigam
    • 2
  1. 1.Staatliches Museum für Naturkunde GörlitzGörlitzGermany
  2. 2.Klinikum Görlitz GmbHGörlitzGermany

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