Folia Geobotanica

, Volume 31, Issue 3, pp 315–321 | Cite as

Determination of the ploidy level in the genusHieracium subgenusPilosella (Hill) S.F. Gray by flow cytometric DNA analysis

  • Siegfried Bräutigam
  • Edeltraut Bräutigam
Apomixis and Taxonomy Proceedings of the Symposium held in Pruhonice, Czech Republic 1995; edited by A.J. Richards, J. Kirschner, J. Stepanek & K. Marhold

Abstract

Ploidy levels of 8 taxa of the genusHieracium subgenusPilosella collected from the same locality were determined by means of flow cytometric estimation of relative nuclear DNA content. The DNA anaysis of isolated nuclei stained with propidium iodide was performed with a flow cytometer equipped with an argon-ion laser. The diploid speciesH. lactucella (2n=18) was used as internal standard. As a rule 20 individuals per taxon were analysed. The flow cytometric analysis proved to be a rapid, accurate and sensitive method for screening of ploidy levels. Considering the known cytological data, the following ploidy levels were determined:H. aurantiacum 4x,H. pilosella 4x,H. piloselloides 4x,H. bauhini 5x,H. caespitosum 5x,H. stoloniflorum 4x,H. brachiatum 4x and 6x,H. leptophyton 7x.H. piloselloides, H. bauhini andH. caespitosum yield relative DNA contents which are the exact multiples of the haploid DNA content ofH. lactucella. In the case ofH. pilosella the haploid DNA content is lower than that predicted from the diploid, namely 0.43 instead of the expected 0.5. The haploid 1x DNA contents of bothH. brachiatum andH. leptophyton range between the value found forH. pilosella and those for the other investigated species, confirming their hybrid origin.

Keywords

Apomixis Asteraceae Chromosome number Compositae Hybrid origin Nuclear DNA content 

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References

  1. Bergounioux C. &Brown S.C. (1990): Plant cell cycle analysis with isolated nuclei. In:Darzynkiewicz Z. &Crissman H.A. (eds.),Methods in cell biology 33, Academic Press, New York, pp. 563–573.Google Scholar
  2. Butterfass Th. (1994): Chloroplast numbers per cell and cell volumes in apomicticHieracium polyploids (Asteraceae).Cytologia 59: 231–235.Google Scholar
  3. Dabrowska J. (1992): Chromosome number and DNA content in taxa ofAchillea L. in relation to the distribution of the genus.Prace Bot. Univ. Wroclawsk. 49: 1–84.Google Scholar
  4. Darzynkiewicz Z., Traganos F., Kapuscinski J, Staiano-Coico L. &Melamed M.R. (1984): Accessibility of DNA in situ to various fluorochromes: relationship to chromatin chandes during erythroid differentiation of Friend leukemia cells.Cytometry 5: 355–363.PubMedCrossRefGoogle Scholar
  5. Doležel J. (1991): Flow cytometric analysis of nuclear DNA content in higher plants.Phytochem. Analysis 2: 143–154.CrossRefGoogle Scholar
  6. Doležel J. &Göhde W. (1995): Sex determination in dioecious plantsMelandrium album andM. rubrun using high-resolution flow cytometry.Cytometry 19: 103–106.PubMedCrossRefGoogle Scholar
  7. Gadella Th.W.J. (1984): Cytology and the mode of reproduction of some taxa ofHieracium subgenusPilosella.Proc. Kon. Ned. Akad. Wetensch., C. 87: 387–399.Google Scholar
  8. Gadella Th.W.J. (1992): Notes on some triple and inter-sectional hybrids inHieracium L. subgenusPilosella (sc(Hill) S.F. GrayProc. Kon. Ned. Akad. Wetensch., C. 95: 51–63.Google Scholar
  9. Galbraith D.W. (1990): Flow cytometric analysis of plant genoms. In:Darznnkiewicz Z. &Crissman H.A. (eds.),Methods in cell biology 33, Academic Press, New York, pp. 549–562.Google Scholar
  10. Galbraith D.W., Harkins K.R., Maddox J.M., Ayres N.M., Sharma D.P. &Firoozapy, E. (1983): Rapid flow cytometric analysis of the cell cycle in intact plant tissues.Science 220: 1049–1051.PubMedCrossRefGoogle Scholar
  11. Gottschlich G. (1987):Hieracium. In:Wagenitz G. (ed.):Hegi, Illustrierte Flora von Mitteleuropa 4/4, Ed. 2, Verlag Paul Parey, Berlin, Hamburg, pp. 1437–1451.Google Scholar
  12. Hopping M.E. (1994): Flow cytometric analysis ofActinidia species.New Zealand J. Bot. 32: 85–93.Google Scholar
  13. Májovský J. et al. (1987):Karyotaxonomický prehlad flóry Slovenska (Karyological survey of the flora of Slovakia). VEDA, VSAV, Bratislava.Google Scholar
  14. Merxmüller H. (1975): Diploide Hieracien.Anales Inst Bot. Cavanilles 32: 189–196.Google Scholar
  15. Nägeli C.W. &Peter A. (1885):Die Hieracien Mittel-Europas 1, R. Oldenbourg, München.Google Scholar
  16. Pogan E. &Wcisło E. (1989): Cytological investigations onHieracium pilosella L. from Poland I. Karyological studies.Acta Biol. Cracov., Ser. Bot. 31: 19–28.Google Scholar
  17. Skalińska M. (1967): Cytological analysis of someHieracium species, subg.Pilosella from mountains of southern Poland.Acta Biol. Cracov., Ser. Bot 10: 127–141.Google Scholar
  18. Turesson B. (1972): Experimental studies inHieracium pilosella L. II. Taxonomy and differentiation.Bot. Not. 125: 223–240.Google Scholar
  19. Zahn K.H. (1922–1930):Hieracium. In:Ascherson P. &Graebner P. (eds.),Synopsis der Mitteleuroäischen Flora 12/1, Gebrüder Bornträger, Leipzig.Google Scholar

Copyright information

© Institute of Botany 1996

Authors and Affiliations

  • Siegfried Bräutigam
    • 1
  • Edeltraut Bräutigam
    • 2
  1. 1.Staatliches Museum für Naturkunde GörlitzGörlitzGermany
  2. 2.Klinikum Görlitz GmbHGörlitzGermany

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