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Molecular and Chemical Neuropathology

, Volume 28, Issue 1–3, pp 285–293 | Cite as

Novel mitochondrial proteins and decreased intrinsic activity of cytochrome-C Oxidase

Characteristics of swayback disease in sheep
  • Trevor Alleyne
  • Andrew Adogwa
  • Anthony Lalla
  • Jerome Joseph
  • Roger John
Part XII Clinical and Experimental Investigations in the Caribbean

Abstract

Swayback disease (SD), a fatal neurological disorder affecting lambs and kids, is characterized by abnormal mitochondria and low cytochrome-c oxidase activity. The cytochrome-c oxidase content and activity and the protein composition of mitochondria isolated from the brains of SD lambs were investigated. Difference spectra analysis indicated that the cytochrome-c oxidase content of mitochondria from SD animals was lower than normal, and electrophoresis showed that when compared to mitochondria from normal animals, lipid-depleted mitochondria from SD lambs had a different protein composition, particularly, in the 40–55 kDa region. Polarographic studies, using cytochrome-c as substrate, confirmed low intrinsic activity of cytochrome-c oxidase within the mitochondria of SD lambs. These studies also showed that at low ionic strength, such mitochondria did not yield the expected characteristic biphasic Eadie-Hofstee plots.

Index Entries

Swayback mitochondria protein composition cytochrome-c oxidase content cytochrome-c oxidase activity biphasicity 

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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Trevor Alleyne
    • 1
  • Andrew Adogwa
    • 1
  • Anthony Lalla
    • 1
  • Jerome Joseph
    • 1
  • Roger John
    • 1
  1. 1.Department of Biochemistry, Faculty of Medical SciencesThe University of the West IndiesSt. AugustineTrinidad and Tobago

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