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Folia Microbiologica

, Volume 41, Issue 3, pp 254–256 | Cite as

Two alternative pathways for substrate assimilation byMucor circinelloides

  • G. Aggelis
Papers

Abstract

Transition of an oleaginous strain ofMucor circinelloides from mycelial to yeast-like form was studied in conditions favoring lipogenesis. In culture media with citric acid as the sole carbon source at concentrations of 7.5 and 10 g/L (C/N ratio of 26 and 35, respectively), the mold accumulated significant quantities of lipid. At a higher citric acid concentration liposynthesis was inhibited and the fermentation mechanism decreased the high substrate concentration in the culture media. Under these conditions yeast-like morphogenesis was observed. In the yeast-like cells, biosynthesis of linoleic and γ-linolenic acid was inhibited. In contrast, no significant changes were observed in the biosynthesis of palmitoleic acid whereas the concentration of oleic acid was increased in the storage lipids of yeast-like cells.

Keywords

Citric Acid Cellular Lipid High Substrate Concentration Citrate Concentration Mycelial Form 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. AFNOR:Recueil de normes Francaises des corps gras—graines oleagineuses et produits derives. 3eme ed. NF T 60-233, p. 95 (1984).Google Scholar
  2. Aggelis G., Ratomahenina R., Arnaud A., Galzy P., Martin-Privat P., Perraud J.P., Pina M., Graille J.: Etude de l'influence des conditions de culture sur la teneur en acide gamma linolenique de souches deMucor.Oleagineux 43, 311–318 (1988).Google Scholar
  3. Aggelis G.: Etude de la production d'acide gamma linolenique par de Phycomycetes (Mucorales).PhD Thesis. Université de Montpellier II—USTL 1989.Google Scholar
  4. Aggelis G., Komaitis M., Papanikolaou S., Papadopoulos G.: A mathematical model for the study of lipid accumulation in oleaginous microorganisms. II. Study of cellular lipids ofMucor circinelloides during growth on a vegetable oil.Grasas y Aceites 46, 245–250 (1995).Google Scholar
  5. Bartnicki-Garcia S.: Control of dimorphism inMucor by hexoses inhibition of hyphal morphogenesis.J. Bacteriol. 96, 1586–1594 (1968).PubMedGoogle Scholar
  6. Beutler H.-O., Michal G.: Neue Methode zur enzymatischen Bestimmung von Äthanol in Lebensmitteln.Z. Anal. Chem. 284, 113–117 (1977).CrossRefGoogle Scholar
  7. Evans C.T., Ratledge C.: The physiological significance of citric acid in the control of metabolism in lipid accumulating yeasts.Biotechnol. Genet. Eng. Rev. 3, 349–374 (1985).Google Scholar
  8. Folch J., Lees M., Sloan-Stanley G.H.: A simple method for the isolation and purification of total lipid from animal tissues.J. Biol. Chem. 226, 497–509 (1957).PubMedGoogle Scholar
  9. Gordon P.A., Stewart P.R., Clark-Walker G.D.: Fatty acid and sterol composition ofMucor genevensis in relation to dimorphism and anaerobic growth.J. Bacteriol. 107, 114–120 (1971).PubMedGoogle Scholar
  10. Holdsworth J.E., Ratledge C.: Lipid turnover in oleaginous yeasts.J. Gen. Microbiol. 134, 339–346 (1988).Google Scholar
  11. Mollering H., Gruger W.: Determination of citrate with citrate lyase.Anal. Biochem. 17, 369–376 (1966).CrossRefGoogle Scholar
  12. Storck R., Morrill R.C.: Respiratory-deficient, yeast-like mutant ofMucor.Biochem. Genet. 5, 467–479 (1971).PubMedCrossRefGoogle Scholar
  13. Terenzi H.F., Storck R.: Stimulation of fermentation and yeast-like morphogenesis inMucor rouxii by phenethyl alcohol.J. Bacteriol. 97, 1248–1261 (1969).PubMedGoogle Scholar

Copyright information

© Folia Microbiologica 1996

Authors and Affiliations

  • G. Aggelis
    • 1
  1. 1.Department of Agricultural Biology and BiotechnologyAgricultural University of AthensAthensGreece

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