Folia Microbiologica

, Volume 37, Issue 4, pp 295–298 | Cite as

DNA-DNA hybridization of some root-nodule bacteria indigenous in the salt-affected soils of egypt

  • H. H. Zahran
Papers
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Abstract

The DNA-DNA hybridization was used to characterize thirty isolates of root-nodule bacteria indigenous to the salt-affected soils of Egypt. Total DNA from different bacterial isolates lacked homology with total DNA probes of the effective strains ofRhizobium leguminosarum andR. meliloti. It is suggested that the genomic structure of the root-nodule bacteria may be modified by salt stress and/or that the effective strains of these bacteria are to be eliminated from the salt-affected soil.

Keywords

Effective Strain Rhizobium Leguminosarum Plasmid Profile Restriction Fragment Length Poly Salty Soil 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Brown G., Khan Z., Lifshitz R.: Plant growth promoting bacteria: strain identification by restriction fragment length polymorphism.Can. J. Microbiol.36, 242–248 (1990).CrossRefGoogle Scholar
  2. Casella S., Frassinetti S., Lupi F., Squartini A.: Effect of cadmium, chromium and copper on symbiotic and free-livingRhizobium leguminosarum biovartrifolii.FEMS Microbiol. Letters49, 343–347 (1988).CrossRefGoogle Scholar
  3. Cooper J.E., Bjourson A.J., Thompson J.K.: Identification ofLotus rhizobia by direct DNA hybridization of crushed root nodules.Appl. Environ. Microbiol.53, 1705–1707 (1987).PubMedGoogle Scholar
  4. Douka C.E., Apostalakis C.G., Skarloy V.D.: Studies ofRhizobium meliloti isolated from salt-affected soils.Ann. Appl. Biol.88, 457–460 (1978).CrossRefGoogle Scholar
  5. Giller K.E., Mc Grath S.P., Hirsch P.R.: Absence of nitrogen fixation in clover grown on soil subject to long-term contamination with heavy metals is due to survival of only ineffectiveRhizobium.Soil. Biol. Biochem.21, 841–848 (1989).CrossRefGoogle Scholar
  6. Kaijalainen S., Lindström K.: Restriction fragment length polymorphism analysis ofRhizobium galegae strains.J. Bacteriol.171, 5561–5566 (1989).PubMedGoogle Scholar
  7. Mc Grath S.P., Brookes P.C., Giller K.E.: Effects of potentially toxic metals in soil derived from past applications of sewage sludge on nitrogen fixation byTrifolium repens L.Soil Biol. Biochem.20, 415–424 (1988).CrossRefGoogle Scholar
  8. Mohammad H.A.: Physiological and ecological studies of some microorganisms isolated from salty soil in Egypt.MSc Thesis. Assiut University, Quena Branch, Quena (Egypt) 1990.Google Scholar
  9. Pepper I.L., Josephson K.L., Nautiyal C.S., Bourque D.P.: Strain identification of highly-competitive bean rhizobium isolated from root nodules: use of fluorescent antibodies, plasmid profiles and gene probes.Soil Biol. Biochem.21, 749–753 (1989).CrossRefGoogle Scholar
  10. Richardson A.E., Simpson R.J., Djordjevic M.A., Rolfe B.G.: Expression of nodulation genes inRhizobium leguminosarum biovartrifolii is affected by low pH and by Ca and Al ions.Appl. Environ. Microbiol.54, 2541–2548 (1988).PubMedGoogle Scholar
  11. Soberon-Chavéz G., Najéra R.: Isolation from soil ofRhizobium leguminosarum lacking symbiotic information.Can. J. Microbiol.35, 464–468 (1989).CrossRefGoogle Scholar
  12. Walker J., Dougan G.: DNA probes: a new role in diagnostic microbiology.J. Appl. Bacteriol.67, 229–238 (1989).PubMedGoogle Scholar
  13. Whiley R.A., Hardie J.M.: DNA—DNA hybridization studies and phenotypic characteristics of strains within the “Streptococcus milleri” group.J. Gen. Microbiol.135, 2623–2633 (1989).PubMedGoogle Scholar
  14. Zahran H.H.: Conditions for successfulRhizobium—legume symbiosis in saline environments.Biol. Fert. Soils12, 73–80 (1991a).CrossRefGoogle Scholar
  15. Zahran H.H.: Cultural and physiological properties of some root nodule bacteria indigenous in the salt-affected soils of Egypt.Bull. Fac. Sci. Assiut Univ.20, 85–99 (1991b).Google Scholar
  16. Zahran H.H.: Characterization of root-nodule bacteria indigenous in the salt-affected soils of Egypt by lipopolysaccharide, protein and plasmid profiles.J. Basic Microbiol.,32, in press (1992).Google Scholar

Copyright information

© Folia Microbiologica 1992

Authors and Affiliations

  • H. H. Zahran
    • 1
  1. 1.Department of BotanyFaculty of ScienceBeni SuefEgypt

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