In Vitro

, Volume 18, Issue 6, pp 531–537 | Cite as

Cholera toxin stimulation of human mammary epithelial cells in culture

  • Martha R. Stampfer


Addition of cholera toxin to human mammary epithelial cultures derived from reduction mammoplasties and primary carcinomas greatly stimulated cell growth and increased the number of times the cells could be successfully subcultured. Other agents known to increase intracellular cAMP levels were also growth stimulatory. The increased growth potential conferred by cholera toxin enhances the usefulness of this cell culture system.

Key words

cholera toxin human mammary epithelial cells 


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  1. 1.
    Stampfer, M.; Hallowes, R. C.; Hackett, A. J. Growth of normal human mammary cells in culture. In Vitro 16: 415–425; 1980.PubMedCrossRefGoogle Scholar
  2. 2.
    Green, H. Cyclic AMP in relation to proliferation of the epidermal cell: a new view. Cell 15: 801–811; 1978.PubMedCrossRefGoogle Scholar
  3. 3.
    Taylor-Papadimitriou, J.; Purkis, P.; Fentiman, I. S. Cholera toxin and analogues of cyclic AMP stimulate the growth of cultured human mammary epithelial cells. J. Cell. Physiol. 101: 317–321; 1980.CrossRefGoogle Scholar
  4. 4.
    Yang, J.; Guzman, R.; Imagawa, W.; McCormick, K.; Nandi, S. Growth factor and cyclic nucleotide induced proliferation of normal and malignant mammary epithelial cells in primary culture. Endocrinology 107: 35–41; 1980.PubMedCrossRefGoogle Scholar
  5. 5.
    Yang, J.; Guzman, R.; Richards, J.; Jentoft, F.; DeVault, M. R.; Wellings, S.; Nandi, S. Primary culture of human mammary epithelial cells embedded in collagen gels. J. Natl. Cancer Inst. 65: 337–343; 1980.PubMedGoogle Scholar
  6. 6.
    Yang, J.; Elias, J. J.; Petrakis, N. L.; Wellings, S. R.; Nandi, S. Effects of hormones and growth factors on human mammary epithelial cells in collagen gel culture. Cancer Res. 41: 1021–1027; 1981.PubMedGoogle Scholar
  7. 7.
    Stampfer, M. R.; Vlodavsky, I.; Smith, H. S.; Ford, R.; Becker, F. F.; Riggs, J. Fibronectin production by human mammary cells. J. Natl. Cancer Inst. 67: 253–261; 1981.PubMedGoogle Scholar
  8. 8.
    Owens, R. B.; Smith, H. S.; Nelson-Rees, W. A.; Springer, E. L. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843–849; 1976.PubMedGoogle Scholar
  9. 9.
    Hackett, A. J.; Smith, H. S.; Springer, E. L.; Owens, R. B.; Nelson-Rees, W. A.; Riggs, J. L.; Gardner, M. B. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (HS578T) and the diploid myoepithelial (HS57Bst) cell lines. J. Natl. Cancer Inst. 58: 1795–1806; 1977.PubMedGoogle Scholar
  10. 10.
    Gill, D. M. Mechanisms of action of cholera toxin. Adv. Cyclic Nucleotide Res. 8: 85–118; 1977.PubMedGoogle Scholar
  11. 11.
    Lechner, J. F.; Haugen, A.; Autrup, H.; McClenden, I. A.; Trump, B. F.; Harris, C. C. Clonal growth of epithelial cells from normal adult human bronchus. Cancer Res. 41: 2294–2304; 1981.PubMedGoogle Scholar
  12. 12.
    Smith, H. S.; Lan, S.; Ceriani, R.; Hackett, A. J.; Stampfer, M. Clonal proliferation of cultured nonmalignant and malignant human breast epithelia. Cancer Res. 41: 4637–4643; 1981.PubMedGoogle Scholar
  13. 13.
    Kung, W.; Bechtel, E.; Geyer, E. Altered cyclic nucleotides cyclic AMP phosphodiesterase and adenyl cyclase activity in dysplastic and neoplastic human mammary tissue. FEBS Lett. 82: 102–105; 1977.PubMedCrossRefGoogle Scholar
  14. 14.
    Minton, J. P.; Foecking, M. K.; Webster, D. J. T.; Matthews, R. H. Caffeine, cyclic nucleotides and breast disease. Surgery 86: 105–109; 1979.PubMedGoogle Scholar
  15. 15.
    Stampfer, M. R.; Bartholomew, J. C.; Smith, H. S.; Bartley, J. Metabolism of benzo(a)pyrene by human mammary epithelial cells: toxicity and DNA adduct formation. Proc. Natl. Acad. Sci. USA 78: 6251–6256; 1981.PubMedCrossRefGoogle Scholar
  16. 16.
    Bartley, J.; Bartholomew, J. C.; Stampfer, M. R. Metabolism of benzo(a)pyrene by human epithelial and fibroblastic cells: metabolite patterns and DNA adduct formation. J. Cell Biochem. 18: 135–148; 1982.PubMedCrossRefGoogle Scholar
  17. 17.
    Smith, H. S.; Hackett, A. J.; Lan, S.; Stampfer, M. R. Use of an efficient method for culturing human mammary epithelial cells to study adriamycin sensitivity. Cancer Chemother. Pharmacol. 6: 237–244; 1981.PubMedCrossRefGoogle Scholar

Copyright information

© Tissue Culture Association, Inc 1982

Authors and Affiliations

  • Martha R. Stampfer
    • 1
    • 2
  1. 1.Peralta Cancer Research Institute, Paralta HospitalOakland
  2. 2.Donner Laboratory, Lawrence Berkeley LaboratoryBerkeley

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