Folia Geobotanica

, Volume 40, Issue 4, pp 407–419 | Cite as

A first approach to the molecular phylogeny of the genusEchinops (Asteraceae): Sectional delimitation and relationships with the genusAcantholepis

  • Teresa Garnatje
  • Alfonso Susanna
  • Núria Garcia-Jacas
  • Roser Vilatersana
  • Joan Vallès
Article

Abstract

A first attempt to establish the phylogeny of the generaEchinops andAcantholepis has been carried out using the analysis of the internal transcribed spacers (ITS) sequences of the nuclear ribosomal DNA including 30Echinops species and the only species of the monotypicAcantholepis. The results of this analysis are discussed in the light of morphological and cytogenetic characters. The genusAcantholepis is placed in a robust clade withEchinops nanus, and together they appear in a basal position to other members ofEchinops. The ITS phylogeny and several other characters, such as chromosome number and nuclear DNA amount, do not agree with the sections currently recognized withinEchinops. Some groups are defined in the present approach, but further studies are necessary to reach a complete, stable and natural infrageneric classification of this genus.

Keywords

Compositae ITS Nuclear ribosomal DNA 

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References

  1. Álvarez I. &Wendel J.F. (2003): Ribosomal ITS sequences and plant phylogenetic inference.Molec. Phylogenet. Evol. 29: 417–434.PubMedCrossRefGoogle Scholar
  2. Bentham G. (1873):Compositae. In:Bentham G. &Hooker J.D. (eds.),Genera plantarum 2, Reeve and Co., London, pp. 163–533.Google Scholar
  3. Bennett M.D. (1972): Nuclear DNA amount and minimum generation time in herbaceous plants.Proc. Roy. Soc. London B 191: 109–135.CrossRefGoogle Scholar
  4. Bremer K. (1988): The limits of amino acid sequence data in angiosperm phylogenetic reconstruction.Evolution 42: 795–803CrossRefGoogle Scholar
  5. Bremer K. (1994): Asteraceae:Cladistics and classification. Timber Press, Portland.Google Scholar
  6. Candolle A.P. (1837):Prodromus systematis naturalis regni vegetabilis 6. Treuttel et Würtz, Paris.Google Scholar
  7. Davis P.H. (ed.) (1975):Flora of Turkey and the East Aegean Islands 5. University Press, Edinburgh.Google Scholar
  8. Dimitrova D. &Greilhuber J. (2000): Karyotype and DNA-content evolution in ten species ofCrepis (Asteraceae) distributed in Bulgaria.Bot. J. Linn. Soc. 132: 281–297.CrossRefGoogle Scholar
  9. Dittrich M. (1977):Cynareae-Systematic review. In:Heywood V.H., Harborne J.B. &Turner B.L. (eds.),The biology and chemistry of the Compositae 2, Academic Press, London, pp. 999–1015.Google Scholar
  10. Dittrich M. (1996): Die Bedeutung morphologischer und anatomischer Achänen-Merkmale für die Systematik der TribusEchinopeae Cass. undCarlineae Cass.Boissiera 51: 9–102.Google Scholar
  11. Doyle J.J. &Doyle J.L. (1987): A rapid DNA isolation procedure for small quantities of fresh leaf tissue.Phytochem. Bull. 19: 11–15.Google Scholar
  12. Felsenstein J. (1985): Confidence limits on phylogenies: An approach using the bootstrap.Evolution 39: 783–791.CrossRefGoogle Scholar
  13. Garcia S., Sanz M., Garnatje T., Kreitschitz A., McArthur E.D. &Vallès J. (2004): Variation of DNA amount in 47 populations of the subtribeArtemisiinae and related taxa (Asteraceae, Anthemideae): karyological, ecological, and systematic implications.Genome 47: 1004–1014.PubMedCrossRefGoogle Scholar
  14. Garcia-Jacas N., Garnatje T., Susanna A. &Vilatersana R. (2002): Tribal and subtribal delimitation and phylogeny of theCardueae (Asteraceae): a combined nuclear and chloroplast DNA analysis.Molec. Phylogenet. Evol. 22: 51–64.PubMedCrossRefGoogle Scholar
  15. Garnatje T., Vallès J., Garcia S., Hidalgo O., Sanz M., Canela M.A. &Siljak-Yakovlev S. (2004a): Genome size inEchinops L. and related genera (Asteraceae, Cardueae): karyological, ecological and phylogenetic implications.Biol. Cell 96: 117–124.PubMedCrossRefGoogle Scholar
  16. Garnatje T., Vilatersana R., Susanna A., Vallès J. &Siljak-Yakovlev S. (2004b): Contribution to the karyological knowledge ofEchinops L. (Asteraceae, Cardueae) and related genera.Bot. J. Linn. Soc. 145: 337–344.CrossRefGoogle Scholar
  17. Greuter W. (2003): The Euro+Med treatment ofCardueae (Compositae) — generic concepts and required new names.Willdenowia 33: 49–61.Google Scholar
  18. Hoffmann O. (1894):Compositae. In:Engler A. &Prantl K. (eds.),Die natürlichen Pflanzenfamilien, Engelmann, Leipzig, vol. 4, pp. 87–387.Google Scholar
  19. Jäger E.J. (1987): Arealkarten derAsteraceen-Tribus als Grundlage der ökogeographischen Sippencharakteristik.Bot. Jahrb. Syst. 108: 481–497.Google Scholar
  20. Kit Tan K. (1995): Seven new species ofEchinops (Asteraceae).Ann. Bot. Fenn. 32: 117–126.Google Scholar
  21. Kožuharov S. (1976):Echinops L. In:Tutin et al. (eds.),Flora Europaea 4, Cambridge University Press, Cambridge, pp. 212–214.Google Scholar
  22. Kruse J. &Meusel H. (1972): Zur Blattfolge und Blattbildung einiger Cynareen-Gattungen. 2.Acantholepis, Xeranthemum, Chardinia, Siebera, Amphoricarpos. Feddes Repert. Spec. Nov. Regni Veg. 83: 289–308.Google Scholar
  23. Leitch I.J., Chase M.W., Bennett M.D. (1998): Phylogenetic analysis of DNA C-values provides evidence for a small ancestral genome size in flowering plants.Ann. Bot. (Oxford) 82: 85–94.CrossRefGoogle Scholar
  24. Maddison D.R. (1991): The discovery and importance of multiple islands of most parsimonious trees.Syst. Zool. 40: 315–328.CrossRefGoogle Scholar
  25. Mehra P.N., Gill B.S., Mehta J.K. &Sidhu S.S. (1965): Cytological investigations on the IndianCompositae. I. North-Indian taxa.Caryologia 18: 35–68.Google Scholar
  26. Morgan D.R. (1997): Decay analysis of large sets of phylogenetic data.Taxon 46: 509–517.CrossRefGoogle Scholar
  27. Moore R.J. (1973): Index to plant chromosome numbers 1967–1971.Regnum Veg. 90.Google Scholar
  28. Murray B.G., Cameron E.K. &Stardring L.S. (1992): Chromosome numbers, karyotypes, and nuclear DNA variation inPratia Gaudin (Lobeliaceae).New Zealand J. Bot. 30: 181–187.Google Scholar
  29. Nickrent D.L., Schuette K.P. &Starr E.M. (1994): A molecular phylogeny ofArceuthobium (Viscaceae) based on nuclear ribosomal DNA internal transcribed spacer sequences.Amer. J. Bot. 81: 1149–1160.CrossRefGoogle Scholar
  30. Ohri D. (1996): Genome size and polyploidy variation in the tropical hardwood genusTerminalia (Combretaceae).Pl. Syst. Evol. 200: 225–232.CrossRefGoogle Scholar
  31. Petit D.P. (1988): Le genreEchinops L. (Compositae, Cardueae). 1. Position phylétique et interprétation de l’incapitulescence.Candollea 43: 467–481.Google Scholar
  32. Petit D.P. (1997): Generic interrelationships of theCardueae (Compositae): A cladistic analysis of morphological data.Pl. Syst. Evol. 207: 173–203.CrossRefGoogle Scholar
  33. Petit D.P., Mathez J. &Qaid A. (1996): Early differentiation of theCardueae sensu lato, morphology and pollen. In:Hind D.J.N. &Beentje H.J. (eds.),Compositae: Systematics. Proceedings of the International Compositae Conference 1, Kew, 1994, Royal Botanic Gardens, Kew, pp. 79–93.Google Scholar
  34. Pignatti S. (ed.) (1982):Flora d’Italia 3. Edagricole, Bologna.Google Scholar
  35. Rechinger K.H. (ed.) (1979):Flora des Iranischen Hochlandes und der umrahmenden Gebirge. CompositaeIII — Cynareae. Akademische Druck-u. Verlagsanstalt, Graz.Google Scholar
  36. Shishkin B.K. & Bobrov E.G. (eds.) (1997):Flora of the USSR 27. Bishen Singh, Mahendra Pal Singh and Koeltz Scientific Books, Dehra Dun.Google Scholar
  37. Soltis D.E., Soltis P.S., Collier T.G. &Edgerton M.L. (1991): TheHeuchera group (Saxifragaceae): Evidence for chloroplast transfer and paraphyly.Amer. J. Bot. 78: 1091–1112.CrossRefGoogle Scholar
  38. Susanna A. &Garcia-Jacas N. (2005): The tribeCardueae. In:Kubitzki J. (ed.),The families and genera of vascular plants, Springer-Verlag, Heidelberg (in press).Google Scholar
  39. Swofford D.L. (1999):PAUP*. Phylogenetic analysis using parsimony (* and other methods). Version 4. Sinauer, Sunderland.Google Scholar
  40. Tomšovic P. (1997): Some palynological observations on the genusEchinops (Asteraceae) and their taxonomic implications.Preslia 69: 31–33.Google Scholar
  41. Valdés B. (1996): Tres nuevas combinaciones enAsteraceae.Lagascalia 18: 306.Google Scholar
  42. White T.J., Bruns T., Lee S. &Taylor J. (1990): Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In:Innis M., Gelfand D., Sninsky J. &White T. (eds.),PCR protocols: A guide to methods and applications, Academic Press, San Diego, pp. 315–322.Google Scholar

Copyright information

© Institute of Botany, Academy of Sciences of the Czech Republic 2005

Authors and Affiliations

  • Teresa Garnatje
    • 1
  • Alfonso Susanna
    • 1
  • Núria Garcia-Jacas
    • 1
  • Roser Vilatersana
    • 1
  • Joan Vallès
    • 2
  1. 1.Institut Botànic de Barcelona (CSIC-Ajuntament de Barcelona)Barcelona, CataloniaSpain
  2. 2.Laboratori de Botànica, Facultat de FarmàciaUniversitat de BarcelonaBarcelona, CataloniaSpain

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