Folia Geobotanica

, Volume 36, Issue 3, pp 303–320 | Cite as

A taxonomic study of theVaccinium sect.Oxycoccus (Hill) W.D.J. Kock (Ericaceae) in the Czech Republic and adjacent territories

  • Jan Suda
  • Martin A. Lysák


The ploidy level of plants of theVaccinium sect.Oxycoccus (Hill)W.D.J. Koch sampled in the Czech Republic, Germany, Austria and Poland was determined by chromosome counting and/or by flow cytometry. Forty-five characters were measured and scored in the morphometric analysis. Principal component analysis, cluster analysis, canonical discriminant analysis and classificatory discriminant analysis were used in the statistical analyses.

Diploid (2n=24), tetraploid (2n=48) and hexaploid (2n=72) populations were confirmed and a new ploidy level — pentaploid hybrid plants (2n=60) — was revealed. Results of the multivariate morphometric analysis support the separation of the two native species. DiploidV. microcarpum (Turcz. exRupr.)Schmalh. differs from the polyploids by smaller size of petals, shorter style and stamens (stamens have long filaments and short anthers), glabrous pedicels, mostly solitary flowers, earlier flowering and by occurrence predominantly inPolytrichum strictum tufts. The low taxonomic significance of some features often used in keys for their separation (shape of fruits, insertion of prophylla, pubescence of filaments) was confirmed.V. oxycoccos L. includes three ploidy levels. The hexaploids represent the most abundant ploidy level in the area studied. They show a slightly bigger size of petals, longer bracts, prophylla, style, sepal tips and wider seeds in comparison with the tetraploids. Pentaploid cranberries are hitherto known only from the Czech Republic. They differ particularly in the low proportion of fully-developed tetrads.


Chromosome numbers Flow cytometry Multivariate morphometrics Polyploidy 


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  1. Ahokas H. (1971a): Cytology of hexaploid cranberry with special reference to chromosomal fibres.Hereditas 68: 123–136.CrossRefGoogle Scholar
  2. Ahokas H. (1971b): Notes on polyploidy and hybridity inVaccinium species.Ann. Bot. Fenn. 8: 254–256.Google Scholar
  3. Ahokas H. (1996): Is the polyploid cranberry (Vaccinium sp.) in Finland tetraploid or hexaploid?Nord. J. Bot. 16: 185–189.Google Scholar
  4. Baumgarten J.C.G. (1816):Enumeratio stirpium magno transsilvaniae principatui. Vindobona.Google Scholar
  5. Bretagnolle F. &Thompson J.D. (1995): Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of autopolyploid plants.New Phytol. 129: 1–22.CrossRefGoogle Scholar
  6. Camp W.H. (1944): A preliminary consideration of the biosystematy ofOxycoccus.Bull. Torrey Bot. Club 71: 426–437.CrossRefGoogle Scholar
  7. Costich D.E., Ortiz R., Meagher T.R., Bruederle L.P. &Vorsa N. (1993): Determination of ploidy level and nuclear DNA content in blueberry by flow cytometry.Theor. Appl. Genet. 86: 1001–1006.CrossRefGoogle Scholar
  8. Čvančcara A. (1990):VacciniaceaeS.F. Gray. In:HejnýS. &SlavíkB. et al. (eds.),Květena ČR 2, Academia, Praha, pp. 503–508.Google Scholar
  9. Darrow G.M., Camp W.H. Fischer H.E. &Dermen H. (1944): Chromosome numbers inVaccinium and related groups.Bull. Torrey Bot. Club 71: 498–506.CrossRefGoogle Scholar
  10. Dayton W.A. (1938): A cranberry from the Tahoe national forest.Madroño 4: 201–203.Google Scholar
  11. Doležel J. (1997): Application of flow cytometry for the study of plant genomes.J. Appl. Genet. 38: 282–302.Google Scholar
  12. Duty J. (1960): Floristische Beiträge zur geobotanischen Geländearbeit in Mitteldeutschland (V). 2.Oxycoccus macrocarpusPers., 3.Oxycoccus microcarpusTurcz.Wiss. Z. Martin-Luther-Univ. Halle-Wittenberg 9: 419–420.Google Scholar
  13. Dweikat I.M. &Lyrene P. M. (1988): Production and viability of unreduced gametes in triploid interspecific blueberry hybrids.Theor. Appl. Genet. 76: 555–559.CrossRefGoogle Scholar
  14. Dyer A.F. (1963): The use of lacto-propionic orcein in rapid squash methods for chromosome preparations.Stain Technol. 38: 85–90.Google Scholar
  15. Eklund O. (1926): Zur Systematik und Verbreitung der GattungOxycoccusHill in Fennoscandia orientalis.Acta Soc. Fauna et Flora Fenn. 55: 1–16.Google Scholar
  16. Galbraith D.W., Lambert G.M., Macas J. &Doležel J. (1998): Analysis of nuclear DNA content and ploidy in higher plants. In:Robinson S.P. et al. (eds.),Current protocols in cytometry, John Wiley & Sons, Inc., New York, pp. 7.6.1–7.6.22.Google Scholar
  17. Grigora I.M. (1984): Ekologo-fitotsenotichni vlastivostiOxycoccus microcarpusTurcz. exRupr. na Ukrains’komu polissi (Ecological and phytocenotic properties ofOxycoccus microcarpusTurcz. exRupr. in the Ukranian Polessie).Ukr. Bot. Zhurn. 41: 24–28.Google Scholar
  18. Gugnacka-Fiedor W. (1983): The variability of phenol compounds based on karyological studies of taxa of the genusVaccinium L., subgenusOxycoccus (Hill)A. Gray.Acta Soc. Bot. Polon. 52: 87–99.Google Scholar
  19. Gugnacka-Fiedor W. (1986): Zmiennosć morfologiczna taksonow rodzajuOxycoccusHill (Morphological variability of taxa of the genusOxycoccusHill).Stud. Soc. Sci. Torun., Sect. D, Bot. 11: 1–57.Google Scholar
  20. Hagerup O. (1928): Morphological and cytological studies of Bicornes.Dansk. Bot. Ark. 6: 1–27.Google Scholar
  21. Hagerup O. (1940): Studies on the significance of polyploidy. IV.Oxycoccus. Hereditas 26: 399–410.CrossRefGoogle Scholar
  22. Kulesza W. (1929):Oxycoccos microcarpaTurcz. w nadlesnictwie nowogrodzkiem (Ein Standort vonOxycoccos microcarpa bei Nowogródek).Acta Soc. Bot. Polon. 6: 81–85.Google Scholar
  23. Lippert W. &Merxmüller H. (1986):Vaccinium macrocarponAiton im Haspelmoor.Ber. Bayer. Bot. Ges. 57: 183–184.Google Scholar
  24. Měsíček J. &Jarolímová V. (1992):List of chromosome numbers of the Czech vascular plants. Academia, Praha.Google Scholar
  25. Ortiz R., Vorsa N., Bruederle L.P. &Laverty T. (1992a): Occurrence of unreduced pollen in diploid blueberry species,Vaccinium sect.Cyanococcus. Theor. Appl. Genet. 85: 55–60.Google Scholar
  26. Ortiz R., Bruederle L.P., Laverty T. &Vorsa N. (1992b): The origin of polyploids via 2n gametes inVaccinium sectionCyanococcus.Euphytica 61: 241–246.CrossRefGoogle Scholar
  27. Popova T.N. (1972):Vaccinium L. In:Tutin T.G. et al. (ed.),Flora Europaea 3, Cambridge University Press, Cambridge, pp. 12–13.Google Scholar
  28. Porsild M.P. (1930): Stray contributions to the flora of Greenland. I–V.Meddel. Grönland 77/1: 38–42.Google Scholar
  29. Ravanko O. (1990): The taxonomic value of morphological and cytological characteristics inOxycoccus (subgenus ofVaccinium, Ericaceae) species in Finland.Ann. Bot. Fenn. 27: 235–239.Google Scholar
  30. SAS Institute (1990a):SAS procedures quide, Version 6. Ed. 3. Cary: SAS Institute.Google Scholar
  31. SAS Institute (1990b):SAS/STAT® user’s guide, Version 6. Ed. 4. Cary: SAS Institute.Google Scholar
  32. Sneath P.H. A. &Sokal R.R. (1973):Numerical taxonomy. W.H. Freeman & Company, San Francisco.Google Scholar
  33. Soltis D.E. &Soltis P.S. (1995): The dynamic nature of polyploid genomes.Proc. Natl. Acad. U.S.A. 92: 8089–8091.CrossRefGoogle Scholar
  34. Suda J. (1998): Taxonomická problematika roduOxycoccus v České Republice se zvláštním zřetelem k území Šumavy (A taxonomic study of the genusOxycoccus in the Czech Republic, especially in the Šumava Mts.).Zprávy Čes. Bot. Společn. 32 (1997): 189–195.Google Scholar
  35. Svensson B.M. (1995): Carbon allocation patterns in two closely related stoloniferousVaccinium species.Acta Ecologica 16: 507–517.Google Scholar
  36. Vander Kloet S.P. (1983): The taxonomy ofVaccinium & Oxycoccus.Rhodora 85: 1–43.Google Scholar
  37. Vorsa N. &Ballington J.R. (1991): Fertility of triploid highbush blueberry.J. Amer. Soc. Hort. Sci. 116: 336–341.Google Scholar
  38. Wenderoth C. &Wenderoth K. (1994): Zur Verbreitung karyologisch untersuchter Moosbeeren (Vaccinium oxycoccus s.l.) in Teilen Mitteleuropas (Mittel-und Süddeutschland sowie Österreich).Ber. Bayer. Bot. Ges. 64: 147–155.Google Scholar

Copyright information

© Institute of Botany, Academy of Sciences of the Czech Republic 2001

Authors and Affiliations

  1. 1.Department of BotanyCharles UniversityPragueCzech Republic
  2. 2.Institute of BotanyAcademy of Sciences of the Czech RepublicPrůhoniceCzech Republic
  3. 3.Laboratory of Molecular Cytogenetics and CytometryInstitute of Experimental BotanyOlomoucCzech Republic

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