Advertisement

Plant Molecular Biology Reporter

, Volume 20, Issue 1, pp 69–69 | Cite as

A simple protocol for RNA isolation from fruit trees containing high levels of polysaccharides and polyphenol compounds

  • Chun Gen Hu
  • Chikako Honda
  • Masayuki Kita
  • Zilian Zhang
  • Tomomi Tsuda
  • Takaya MoriguchiEmail author
Publish by Abstract

Abstract

RNA isolation is a prerequisite to the study of gene expression at the molecular level and has an increasingly important role in physiological and genetic investigations of fruit trees. However, RNA isolation is difficult in fruit trees that contain large amounts of polysaccharides and polyphenol compounds. Until now, no commercial kit has been developed specifically for, the isolation of high-quality RNA from fruit trees. Furthermore, because of the large amounts of polysaccharides and polyphenol compounds, the common protocols for RNA isolation are tedious and usually result in poor yields when applied to kiwifruit, apples, and peaches. Here we describe a simple method for RNA islation from fruit trees. This procedure involves easy grinding by zirconia balls; washing the fruit samples before extraction; extraction of RNA; and removal of proteins, polyphenols, and polysaccharides by insoluble polyvinylpyrrolidone (PVP) and potassium acetate with ethanol. The protocol results in high-quality RNA, as evidenced by performing reverse transcription-polymerase chain reaction (RT-PCR) and northern blot analysis.

Key words

fruit trees polyphenols polysaccharides RNA extraction 

Copyright information

© International Society for Plant Molecular Biology 2002

Authors and Affiliations

  • Chun Gen Hu
    • 1
    • 2
  • Chikako Honda
    • 2
  • Masayuki Kita
    • 2
  • Zilian Zhang
    • 2
  • Tomomi Tsuda
    • 2
  • Takaya Moriguchi
    • 2
    Email author
  1. 1.Department of HorticultureHuazhong Agricultural UniversityWuhan, Hubei ProvincePeople’s Republic of China
  2. 2.Department of Plant, Cell, and EnvironmentNational Institute of Fruit Tree ScienceTsukuba, IbarakiJapan

Personalised recommendations