Advertisement

Endocrine

, Volume 7, Issue 3, pp 337–341 | Cite as

Neonatal exposure to coumestrol, a phytoestrogen, does not alter spermatogenic potential in rats

  • Caleb A. AwoniyiEmail author
  • Dawn Roberts
  • Varadaraj Chandrashekar
  • D. N. Rao Veeramachaneni
  • Bradley S. Hurst
  • Kathleen E. Tucker
  • William D. Schlaff
Original Articles

Abstract

The objective of this study was to determine the effects of neonatal exposure to phytoestrogens on male reproductive function as adults. Male rats were injected either with 100 μg coumestrol or DMSO (controls) daily during their first 5 d of life. Pituitary gland, testes, sex accessory organs, and blood were collected on d 60 of life. Serum testosterone, LH, and FSH levels were determined by RIA. Levels of steady-state mRNA for gonadotrophin subunits (LHβ and FSHβ were determined by Northern blot analysis and quantified by a scanning densitometer. Coumestrol had no effect on weights of testes and sex accessory organs, or sperm count. Similarly, there were no significant differences among serum concentrations of testosterone, LHβ and FSH of coumestrol-treated rats and those of controls. Whereas steady state levels of LHβ mRNA in coumestrol-treated rats did not differ from those of controls, steady state levels of FSHβ mRNA increased (37%) in treated animals. However, the augmented FSHβ mRNA expression in coumestrol-treated rats did not negatively affect reproductive potential in male rats. We conclude that neonatal exposure to coumestrol does not alter reproductive organ structure or spermatogenic potential in male rats.

Key Words

Coumestrol Spermatogenesis Testis Gonadotrophin subunits 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Carlsen, E., Giwereman, A., Keiding, N., and Skakkebaek, N. E. (1992).Brit. Med. J. 305, 609–613.PubMedGoogle Scholar
  2. 2.
    Auger, J., Kunstmann, J. M., Czyglik, F., and Jouannet, P. (1995).N. Eng. J. Med. 332, 281–285.CrossRefGoogle Scholar
  3. 3.
    Pajarinen, J., Laippala, P., Penttila, A., and Karhunen, P. (1997).Br. Med. J. 314, 13–18.Google Scholar
  4. 4.
    Sharpe, R. M. and Skakkebaek, N. E., (1993).Lancet 341, 1392–1395.PubMedCrossRefGoogle Scholar
  5. 5.
    Harborne, J. B. (1979). In:Herbivores: Their Interaction with Secondary Plant Chemicals. Rosenthal, G. A. and Janzen, D. H. (eds) Academic, New York.Google Scholar
  6. 6.
    Bickoff, E. M., Booth, A. N., Lyman, R. L., Livingston, A. L., Thompson, C. R., and Deeds, F. (1957).Science 126, 969–970.PubMedCrossRefGoogle Scholar
  7. 7.
    Livingston, A. L., Bickoff, E. M., Guggolz, J., and Thompson, C. R. (1961).J. Agric. Food. Chem. 9, 135–137.CrossRefGoogle Scholar
  8. 8.
    Fotsis, T. and Adlercreutz, H. (1987).J. Steroid. Biochem. 28, 203–213.PubMedCrossRefGoogle Scholar
  9. 9.
    Medlock, K. L., Branham, W. S., and Sheehan, D. M. (1995).proc. Soc. Exp. Biol. Med. 208, 307–313.PubMedGoogle Scholar
  10. 10.
    Burroughs, C. D., Mills, K. T., and Bern, H. A. (1990).Reprod. Toxicol. 4, 127–135.PubMedCrossRefGoogle Scholar
  11. 11.
    Fredricks G. R., Kincaid, R. L., Bondioli, K. R., and Wright, R. W. Jr. (1981).Proc. Soc. Exp. Bio. Med. 167, 237–241.Google Scholar
  12. 12.
    Borroughs, C. D., Bern, H. A., and Stolcstad, E. L. R. (1985).J. Toxicol. Environ. Health 15, 51–61.CrossRefGoogle Scholar
  13. 13.
    Bibbo, M., Gill, W. B., Azizi, F., Blough, R., Fang, V. S., Rosenfield, R. L., Schumacher, F., Sonek, M. G., and Wied, G. L. (1977).Obstet. Gynecol. 49, 1–8.PubMedGoogle Scholar
  14. 14.
    Gill, W. B., Schumacher, F., and Bibbo, M. (1977).J. Urol. 117, 477–480.PubMedGoogle Scholar
  15. 15.
    Veeramachaneni, D. N. R., Sherman, G. B., Floyd, J. G., Ott, R. S., and Hixon, J. E. (1988).Fund. Appl. Toxicol. 10, 73–81.CrossRefGoogle Scholar
  16. 16.
    Bromwich, P., Cohen, J., Stewart, I., and Walker, A. (1994).Br. Med. J. 309, 1922.Google Scholar
  17. 17.
    Safe, S. H. (1995).Environ. Health Perspect. 103, 346–351.PubMedCrossRefGoogle Scholar
  18. 18.
    Stillman, R. J. (1982).Am. J. Obstet. Gynecol. 142, 905–921.PubMedGoogle Scholar
  19. 19.
    Kuiper G. D. J. M., Carlsson B., Grandien K., Enmark E., Haggblad, J., Nilsson S., et al. (1997).Endocrinology 138, 863–870.PubMedCrossRefGoogle Scholar
  20. 20.
    Adlercreutz, H., Fotsis, T., Heikkinen, R., Dwyer, J. T., Woods, M., Goldin B. R., and Gorbach, S. L. (1982).Lancet 2, 1295–1299.PubMedCrossRefGoogle Scholar
  21. 21.
    Adlercruetz, H. (1990).Scan J. Clin. Lab. Invest. 50, 3–23 (suppl. 201).CrossRefGoogle Scholar
  22. 22.
    Axelson, M., Kirk, D. N., Farrant, R. D., Cooley, G., Lawson, A. M., and Setchell, K. D. R. (1982).Biochem. J. 201, 353–357.PubMedGoogle Scholar
  23. 23.
    Axelson, M., Sjovall, J., Gustafsson, B. E., and Setchell, K. D. R. (1984).J. Endocrinol. 102, 29–56.CrossRefGoogle Scholar
  24. 24.
    Yatani, R., Chigusa, I., Akayaki, K., Stemmerman, G. N., Welsh, R. A., and Correa, P. (1982).Int. J. Cancer 29, 611–616.PubMedCrossRefGoogle Scholar
  25. 25.
    Faber, K. A. and Hughes, C. L. Jr. (1931).Biol. Reprod. 45, 649–653.CrossRefGoogle Scholar
  26. 26.
    Faber, K. A. and Hughes, C. L. Jr. (1933).Reprod. Toxicol. 7, 35–39.CrossRefGoogle Scholar
  27. 27.
    Levy, J. R., Faber, K. A., Ayyash, L., and Hughes, C. L., Jr. (1995).Proc. Soc. Exp. Biol. Med. 208, 60–66.PubMedGoogle Scholar
  28. 28.
    Register B., Bethel, M. A., Thompson, N., Walmer, D., Blohm, P., Ayyash, L., et al. (1995).Proc. Soc. Exp. Bio. Med. 208, 72–77.Google Scholar
  29. 29.
    Steinberger, E. and Duckett, G. E. (1965).Endocrinology 77, 1184–1189.Google Scholar
  30. 30.
    Ewing, L. L., Desjardins, C., Irby, D. C., and Robaire, B. (1977).Nature 269, 409–411.PubMedCrossRefGoogle Scholar
  31. 31.
    Van Beurden, W. M. O., Mulder, E., De Jong F. H., and van der Molen, H. J. (1977).Endocrinology 101, 342–349.PubMedGoogle Scholar
  32. 32.
    Robaire, B., Ewing, L. L., Irby, D. C., and Desjardins, C. (1979).Biol. Reprod. 21, 455–463.PubMedCrossRefGoogle Scholar
  33. 33.
    Huhtaniemi, I. T., Nevo, N., Amsterdam A., and Naor, Z. (1986).Biol. Reprod. 35, 501–509.PubMedCrossRefGoogle Scholar
  34. 34.
    van Den Dungen, H. M., van Dieten, J. A. M. J., van Rees, G. P., and Shoemaker, J. (1990).Life Sci. 46, 1081–1089.PubMedCrossRefGoogle Scholar
  35. 35.
    Robb, G. W., Amann, R. P., and Killiam, G. J. (1978).J. Reprod. Fertil. 54, 103–107.PubMedCrossRefGoogle Scholar
  36. 36.
    Falvo, R. E. and Nalbandov, A. V. (1974).Endocrinology 95, 1466–1468.PubMedGoogle Scholar
  37. 37.
    Erskine, M. S. and Baum, M. J. (1982).Endocrinology 111, 767–772.PubMedGoogle Scholar
  38. 38.
    Niswender, G. D., Reichert, L. E., and Zimmerman, D. R. (1970).Endocrinology 87, 576–580.PubMedCrossRefGoogle Scholar
  39. 39.
    Chandrashekar, V., Bartke, A., and Seller, K. (1987).Endocrinology 120, 758–763.PubMedGoogle Scholar
  40. 40.
    Chomcznski, P. and Sacchi, N. (1987).Anal. Biochem. 162, 156–159.Google Scholar
  41. 41.
    Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989).Molecular Cloning: A Laboratory Manual, NY, Cold Spring Harbor Laboratory Press.Google Scholar
  42. 42.
    Gharib, S. D., Bowers, S. M., Reed, L. R., and Chin, W. W. (1986).J. Clin. Invest. 77, 582–589.PubMedGoogle Scholar
  43. 43.
    Gharib, S. D., Wierman, M. E., Badger, T. M., and Chin, W. W. (1987).J. Clin. Invest. 80, 294–299.PubMedGoogle Scholar
  44. 44.
    Scheffe, H. (1959).The Analysis of Variance. New York, Wiley and Sons.Google Scholar

Copyright information

© Humana Press Inc 1998

Authors and Affiliations

  • Caleb A. Awoniyi
    • 1
    Email author
  • Dawn Roberts
    • 1
  • Varadaraj Chandrashekar
    • 2
  • D. N. Rao Veeramachaneni
    • 3
  • Bradley S. Hurst
    • 1
  • Kathleen E. Tucker
    • 1
  • William D. Schlaff
    • 1
  1. 1.Division of Reproductive Endocrinology, Department of Obstetrics and GynecologyUniversity of Colorado Health Sciences CenterDenver
  2. 2.Department of PhysiologySouthern Illinois UniversityCarbondale
  3. 3.Animal Reproduction and Biotechnology LaboratoryColorado State UniversityFort Collins

Personalised recommendations