Applied Biochemistry and Biotechnology

, Volume 11, Issue 6, pp 433–443

Recycle of the cellulase— enzyme complex after hydrolysis of steam-exploded wood

  • L. S. Clesceri
  • A. P. Sinitsyn
  • A. M. Saunders
  • H. R. Bungay
Original Articles

Abstract

Cellulases can be recovered in high yields by contacting fresh substrate with hydrolysis filtrate and by extraction of spent hydrolysis residue with pH 7 buffer. Recycled enzymes give hydrolysis rates about equal to those with fresh enzymes. Steam-exploded wood (SEW) is washed with water to remove sugars and byproducts from breakdown of hemicellulose, and recycle of enzymes proceeds better if lignin is also removed prior to hydrolysis. Oven drying of SEW interferes with recycle, and the recovery of enzymes is only one-half of that with SEW that is kept moist.

Effectiveness of enzyme recovery depends on the completeness of hydrolysis, as determined by contact time and enzyme concentration. For cost-effective operation, enzyme should not be recovered until appreciable filter paper activity and carboxylmethylcellulase activity appear in the hydrolysate.

Index Entries

Cellulase-enzyme complex, recycle of steam-exploded wood, hydrolysis of cellulase lignin hemicellulose glucose enzyme production fuel-grade alcohol 

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References

  1. 1.
    Sinitsyn, A. P., Bungay, M. L., Clesceri, L. S., and Bungay, H. R. (1983),Appl. Biochem. Biotech. 8, 25.CrossRefGoogle Scholar
  2. 2.
    Technical and Economic Feasibility of Enzyme Hydrolysis for Ethanol Production from Wood, June 1985, New York State Energy Research and Development Authority Report No. 85-9, Albany, NY, pp. 1–6.Google Scholar
  3. 3.
    Lowrey, O. H., Rosenbrough, N. J., Farr, A. L., and Randall, R. J. (1951),J. Biol. Chem. 193, 265.Google Scholar
  4. 4.
    Sedlack, R. I., (1976),Evolution of Unit Operations and Cellulase Characteristics for Application to a Cellulose to Glucose Conversion System, M. S. Thesis, Rensselaer Polytechnic Institute, NY, p. 27.Google Scholar
  5. 5.
    Miller, G. L., (1959),Anal. Chem.,31, 426.CrossRefGoogle Scholar
  6. 6.
    Mandels, M., Medeiros, J. E., Andreotti, R. E., and Bissett, F. H. (1981),Biotechnol. Bioeng.,23, 2009.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc 1985

Authors and Affiliations

  • L. S. Clesceri
    • 1
  • A. P. Sinitsyn
    • 1
    • 2
  • A. M. Saunders
    • 1
  • H. R. Bungay
    • 1
  1. 1.Rensselaer Polytechnic InstituteTroy
  2. 2.Department of ChemistryMoscow State UniversityUSSR

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