Applied Biochemistry and Biotechnology

, Volume 13, Issue 3, pp 231–240 | Cite as

Continuous production of L-aspartic acid

Improvement of productivity by both development of immobilization method and construction of newEscherichia coli strain
  • Ichiro Chibata
  • Tetsuya Tosa
  • Tadashi Sato


For the continuous production ofl-aspartic acid from fumaric acid and ammonia by the action of aspartase, the enzyme extracted fromEscherichia coli orE. coli cells having high aspartase activity were immobilized by various methods.

In 1973 we succeeded in the industrial production ofl-aspartic acid usingE. coli cells immobilized with polyacrylamide gel.

For the improvement of this process, we developed a novel technique using κ-carrageenan as the immobilizing matrix forE. coli cells. Further, EAPc-7 strain, having higher aspartase activity, was contracted from the parentE. coli by continuous cultivation with a definite medium. The aspartase activity was about seven times higher than that of the parent cells. In 1982 we changed from the conventional method to the improved method, using EAPc-7 strain immobilized with κ-carrageenan.

Index Entries

Production, of L-aspartic acid L-aspartic acid, production of immobilized aspartase, in production of L-aspartic acid aspartase, immobilization of aspartase, with polyacrylamide, production of immobilized cells, in production of L-aspartic acid Escherichia coli ATCC 11303, immobilization ofE. coli ATCC 11303 


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Copyright information

© Humana Press Inc. 1986

Authors and Affiliations

  • Ichiro Chibata
    • 1
  • Tetsuya Tosa
    • 2
  • Tadashi Sato
    • 2
  1. 1.Research and Development HeadquartersTanabe Seiyaku Co. Ltd.OsakaJapan
  2. 2.Research Laboratory of Applied BiochemistryTanabe Seiyaku Co. Ltd.OsakaJapan

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