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Applied Biochemistry and Biotechnology

, Volume 14, Issue 2, pp 107–120 | Cite as

A novel immunoassay system and bioseparation process based on thermal phase separating polymers

  • Nobuo Monji
  • Allan S. Hoffman
Original Articles

Abstract

Poly-N-isopropylacrylamide (polyNIPAAm), a water-soluble, thermally precipitating synthetic polymer, has been conjugated together with a monoclonal antibody (MAb) and utilized in a novel separation method for an immunoassay. The PolyMPAAm precipitates out of water above a critical temperature of 31°C, enabling a polymerbound immune complex to be separated from the solution. The principal advantages of this method are that it utilizes a homogeneous incubation for the antigen-antibody reaction, plus, it has the ability to assay large-molecular-weight antigens with sensitivities equivalent to other nonisotopic heterogeneous immunoassays. In addition, since the polymer-immune complex may be reversibly redissolved by cooling, the method may be used both to concentrate the signal and isolate the analyte. This general technique may also be used for a wide variety of separation processes in addition to immunoassays, in which a specific component in a biological fluid, industrial process stream, or body of water is to be isolated for analysis, recovery, or disposal. Thus, product recovery and/or toxin or pollutant removal processes are possible with this methodology.

Index Entries

Immunoassay antibody-polymer conjugates thermal phase separating polymers polyN-isopropyl acrylamide antigen isolation bioseparation competitive enzyme immunoassay sandwich enzyme immunoassay antigen capture fluorescence immunoassay 

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Copyright information

© Humana Press Inc 1987

Authors and Affiliations

  • Nobuo Monji
    • 1
  • Allan S. Hoffman
    • 2
  1. 1.Genetic Systems CorporationSeattle
  2. 2.Center for Bioengineering and Chemical Engineering Dept.FL-20University of WashingtonSeattle

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