Polymeric thiols as enzyme activators of serum creatine phosphokinase
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Abstract
Several hydrophilic polymeric thiols were prepared from aminoactivated polymeric supports by reaction with N-acetylhomocysteinethiolactone. Supports include agaroses, cellulose, Glycophase™ controlled-pore glass, and Matrex™ acrylic beads. Thiol content in these polymers was 3–72 μmol SH/g dry polymer. Several were effective solid-phase activators of the sulfhydryl-dependent enzyme creatine phosphokinase at concentrations comparable to that of monomeric thiol required for enzyme activation. The kinetic activation curves for the polymeric and the monomeric (thioglucose) activators were similar, suggesting unhindered interaction of the enzyme with the polymeric activator.
Index entries
Polymeric thiols polymeric mercaptans creatine phosphokinase solid-phase enzyme activators sulfhydryl-dependent enzymesPreview
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References
- 1.Lee, C. D. S. and Daly, W. H. (1974),Fortschr. Hochpolym. Forsch. 15, 61.Google Scholar
- 2.Cuatrecacas, P. (1970),J. Biol. Chetn. 245, 3059.Google Scholar
- 3.Axen, R., Drevin, H., and Carlsson (1975),Acta Chem. Scand. B29, 471.CrossRefGoogle Scholar
- 4.Eppenberger, H. M., et al. (1968),J. Biol. Chem. 242, 204; Hangaard, H. (1967),Physiol. Rev. 48, 311.Google Scholar
- 5.Ellman, G. L. (1959),Arch. Biochem. Biophys. 82, 70.CrossRefGoogle Scholar
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© Humana Press Inc. 1987