In Vitro

, Volume 12, Issue 8, pp 571–579 | Cite as

The measurement of spatial precursor distributions in cell culture

  • J. Carlsson
  • H. Lundqvist
  • J. Pontén
Article

Summary

The distribution of incorporated radioactive precursors, for both DNA and protein synthesis, has been measured with a resolution of about 1 mm in cell cultures, using a scanning technique. Either γ radiation and X-rays or β radiation (electrons) were detected by scintillation detectors. Spectrophotometer measurements with a resolution of 1 mm gave good estimates of cell density changes. Glioma cell colonies were used to compared this technique with autoradiography. Variables such as the density of labelled cells and percentage of labelled cells could be estimated rather accurately. For example, an increased cell density was correlated to a local decrease in DNA synthesis.

Key words

automatic detection cell culture cell density DNA-synthesis protein-synthesis radionuclide detection 
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References

  1. 1.
    Pontén, J. 1973. Aging properties of human glia. Inserm. 27: 53–64.Google Scholar
  2. 2.
    Prusoff, W. E. 1963. A review of some aspects of 5-Iododeoxyuridine and Azauridine. Cancer Res. 23:1246.PubMedGoogle Scholar
  3. 3.
    Westermark, B., J. Pontén, and R. Hugosson. 1973. Determinants for the establishment of permanent tissue culture lines from human gliomas. Acta Pathol. Microbiol. Scand. [A]81: 791–805.Google Scholar
  4. 4.
    Ertl, H. H., L. E. Feinendegen, and H. J. Heiniger. 1970. Iodine-125, a tracer in cell biology: physical properties and biological aspects. Phys. Med. Biol. 15: 447–456.PubMedCrossRefGoogle Scholar
  5. 5.
    Lederer, C. M., J. M. Hollander, and I. Perlman. 1968.Table of Isotopes, 6th ed. John Wiley, New York, p. 171.Google Scholar
  6. 6.
    Orvis, A. L. 1967. Systems for data accumulation and presentation. In: G. J. Hine (Ed. 1),Instrumentation in Nuclear Medicine. Volume I. Academic Press, New York, pp. 119–161.Google Scholar
  7. 7.
    Price, J. W. 1964.Nuclear Radiation Detection. McGraw-Hill, New York, pp. 49–50, 394–402.Google Scholar
  8. 8.
    Randerath, K. 1970. An evaluation of film detection methods for weak beta-emitters, particularly tritium. Anal. Biochem. 34: 188–205.PubMedCrossRefGoogle Scholar
  9. 9.
    Cleaver, J. E. 1967.Thymidine Metabolism and Cell Kinetics. North Holland Company, Amsterdam, pp. 70–103.Google Scholar
  10. 10.
    LeMevel, B., R. K. Oldham, S. A. Wells, and R. B. Herberman. 1973. An evaluation of125I-Iododeoxyuridine as a cellular label for in vitro assays: kinetics of incorporation and toxicity. J. Natl. Cancer Inst. 51: 1551–1558.Google Scholar
  11. 11.
    Cleaver, J. E. 1967. Thymidine metabolism and cell kinetics. North Holland Company, Amsterdam, pp. 37–40.Google Scholar
  12. 12.
    Benes, L., E. Rotreklova, and J. Filkuka. 1969. Decrease in tritium activity in3H-thymidine labelled cells after feulgen hydrolysis. Folia Biol. 15: 216–223.Google Scholar
  13. 13.
    Åsard, P. E. 1972. Beta and gamma radiochromatography with spark chambers. Acta Radiol. [Diag.] [Suppl.] (Stockh) 313: 278–285.Google Scholar

Copyright information

© Tissue Culture Association 1976

Authors and Affiliations

  • J. Carlsson
    • 1
  • H. Lundqvist
    • 1
  • J. Pontén
    • 2
    • 1
  1. 1.Department of Physical Biology, The Gustaf Werner InstituteUniversity of UppsalaUppsalaSweden
  2. 2.Department of PathologyUniversity of UppsalaUppsalaSweden

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