Elemental analysis using differential absorption techniques

  • H. Rarback
  • F. Cinotti
  • C. Jacobsen
  • J. M. Kenney
  • J. Kirz
  • R. Rosser
Localization of Trace Elements

Abstract

X-ray differential absorption microanalysis is presented as a technique for trace element analysis of hydrated biological specimens of about 0.1–5 μm thickness. For the study of the light elements (Z≲20), the absorption technique minimizes the radiation dose and, thus, damage to such specimens when compared with X-ray fluorescence. A Scanning Transmission X-ray Microscope (SXTM) is described, which has been used to map the concentration of calcium in bone with better than 300 nm spatial resolution and a sensitivity to 5% calcium by weight. Future plans are briefly discussed that offer the hope of achieving 0.1% trace element sensitivity and 75 nm spatial resolution.

Index Entries

X-ray absorption microanalysis X-ray microscopy biological microanalysis calcium distribution bone 

References

  1. 1.
    J. Kirz, inScanning Electron Microscopy II, SEM, Chicago, IL, 1980, pp. 239–249.Google Scholar
  2. 2.
    C. J. Sparks, inSynchrotron Radiation Research, S. Doniach and H. Winick, eds., Plenum, New York, NY, 1980, pp. 459–509.Google Scholar
  3. 3.
    P. Horowitz and J. A. Howell,Science 178, 608 (1972).PubMedCrossRefGoogle Scholar
  4. 4.
    J. Kirz, inUltrasoft X-Ray Microscopy: Its Application to Biological and Physical Sciences, D. Parsons, ed.,Ann. New York Acad. Sci. 342, 273 (1980).Google Scholar
  5. 5.
    H. Rarback, J. M. Kenney, J. Kirz, M. R. Howells, P. Chang, P. J. Coane, R. Feder, P. J. Houzego, D. P. Kern, and D. Sayre, inX-Ray Microscopy, G. Schmahl and D. Rudolf, eds., Springer-Verlag, Berlin, 1984, pp. 203–215.Google Scholar
  6. 6.
    J. M. Kenney, C. Jacobsen, J. Kirz, H. Rarback, F. Cinotti, W. Thomlinson, R. Rosser, and G. Schidlovsky,J. Microscopy,137, 321 (1985).Google Scholar
  7. 7.
    J. M. Kenney, J. Kirz, H. Rarback, M. R. Howells, P. Chang, P. J. Coane, R. Feder, P. J. Houzego, D. P. Kern, and D. Sayre,Nucl. Inst. Meth. 222, 37 (1984).CrossRefGoogle Scholar
  8. 8.
    The zone plate used for this work was fabricated at the IBM T. J. Watson Research Center, Yorktown Heights, NY. For details,see D. Kernet al. J. Vac. Sci. Technol. B1, 1096 (1983).CrossRefGoogle Scholar
  9. 9.
    J. M. Kenney,Elemental Analysis using a Scanning Transmission X-ray Microscope: Mapping Calcium Concentration in Human Bone, Ph. D Dissertation, SUNY at Stony Brook, NY (1985).Google Scholar
  10. 10.
    M. W. D. Mansfield,Proc. Royal Soc. London A348, 143 (1976).CrossRefGoogle Scholar
  11. 11.
    J. P. Connerade,Contemp. Phys. 19, 415 (1978).CrossRefGoogle Scholar

Copyright information

© The Humana Press Inc 1987

Authors and Affiliations

  • H. Rarback
    • 1
  • F. Cinotti
    • 1
  • C. Jacobsen
    • 2
  • J. M. Kenney
    • 2
  • J. Kirz
    • 2
  • R. Rosser
    • 2
  1. 1.National Synchrotron Light SourceBrookhaven National LaboratoryUpton
  2. 2.Department of PhysicsSUNYStony Brook
  3. 3.King’s CollegeLondonEngland

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