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In Vitro

, Volume 12, Issue 3, pp 180–191 | Cite as

Human colonic adenocarcinoma cells

I. Establishment and description of a new line
  • Baldwin H. Tom
  • Lynne P. Rutzky
  • Milda M. Jakstys
  • Ryoichi Oyasu
  • Celia I. Kaye
  • Barry D. Kahan
Article

Summary

A series of human colonic epithelial cell lines have been cultured from a single patient: LS-180 the original adenocarcinoma, LS-174T a trypsinized variant, and normal colonic tissue. The malignant cells, 20 to 40 µm in diameter and oval to polygonal, exhibited characteristics of normal colonic mucosal cells, namely, abundant microvilli prominent in secretory cells, and the presence of intracytoplasmic mucin vacuoles. The cultured adenocarcinoma cells, but not normal, demonstrated neoplastic properties by producing high levels of carcinoembryonic antigen (CEA) and by the ability to be propagated in hamster cheek pouches and in immunodeprived mice. The CEA production by the newly established line LS-180 released 900 times more CEA per cell into the culture medium and bore 30 times more cell-associated material than the established line, HT-29. These cell lines may permit detection of distinctive chemical, physiological, pharmacologic, and immunologic characteristics of neoplastic colonic cells.

Key words

human cancer colon cell line morphology carcinoembryonic antigen light and electron microscopy 

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References

  1. 1.
    Hellstrom, K. E., and I. Hellstrom. 1974. Lymphocyte-mediated cytotoxicity and blocking serum activity to tumor antigens. Adv. Immunol. 18: 209–277.PubMedCrossRefGoogle Scholar
  2. 2.
    Pellis, N. R., and B. D. Kahan. Tumor-specific neoantigens associated with methylcholanthrene-induced sarcomas. In: H. Busch (Ed.),Methods in Cancer Research. Volume 11. Academic Press, New York. (In Press.)Google Scholar
  3. 3.
    Tompkins, W. A. F., A. M. Watrach, J. D. Schmale, R. M. Schultz, and J. A. Harris. 1974. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J. Natl. Cancer Inst. 52: 1101–1110.PubMedGoogle Scholar
  4. 4.
    Moore, G. E., D. Mount, G. Tara, and N. Schwartz. 1963. Growth of human tumor cells in suspension cultures. Cancer Res. 23: 1735–1741.PubMedGoogle Scholar
  5. 5.
    Egan, M. L., and C. W. Todd. 1972. Carcinoembryonic antigen: synthesis by a continuous line of adenocarcinoma cells. J. Natl. Cancer Inst. 49: 887–889.PubMedGoogle Scholar
  6. 6.
    Egan, M., J. Lautenschleger, J. Coligan, and C. Todd. 1972. Radioimmune assay of carcinoembryonic antigen. Immunochemistry 9: 289–299.PubMedCrossRefGoogle Scholar
  7. 7.
    Gold, P., J. Krupey, and H. Ansari. 1970. Position of the carcinoembryonic antigen of the human digestive system in ultrastructure of tumor cell surface. J. Natl. Cancer Inst. 45: 219–225.PubMedGoogle Scholar
  8. 8.
    Terry, W. D., P. A. Henkart, J. E. Coligan, and C. W. Todd. 1974. Carcinoembryonic antigen: characterization and clinical applications. Transplant. Rev. 20: 100–129.PubMedGoogle Scholar
  9. 9.
    Baldwin, R. W., M. J. Embleton, J. S. P. Jones, and M. J. S. Langman, 1973. Cell-mediated and humoral immune reactions to human tumors. Int. J. Cancer 12: 73–83.PubMedCrossRefGoogle Scholar
  10. 10.
    Baldwin, R. W., M. J. Embleton, and M. R. Price. 1973. Inhibition of lymphocyte cytotoxicity for human colon carcinoma by treatment with solubilized tumor membrane fractions. Int. J. Cancer 12: 84–92.PubMedCrossRefGoogle Scholar
  11. 11.
    Hellstrom, I., and K. E. Hellstrom. 1972. Newer concepts of cancer of the colon and rectum: cellular immunity to human colonic carcinomas. Dis. Colon Rectum 15: 100–105.PubMedCrossRefGoogle Scholar
  12. 12.
    Fogh, J., and G. Trempe. 1975. New Human Tumor Cell Lines. In: J. Fogh (Ed.),Human Tumor Cells In Vitro. Plenum Publishing Corp., New York, pp. 115–154.Google Scholar
  13. 13.
    Vesely, P., and A. Boyde. 1973. The significance of SEM evaluation of the cell surface for tumor cell biology. In:Scanning Electron Microscopy. Part III, IITRI, Chicago, pp. 689–696.Google Scholar
  14. 14.
    David, G. E., and B. S. Williamson. 1971. Amplitude-contrast microscopy in histochemistry. Histochem. 27: 1–20.Google Scholar
  15. 15.
    Revel, J. P., P. Hoch, and D. Ho. 1974. Adhesion of culture cells to their substratum. Exp. Cell Res. 84: 207–218.PubMedCrossRefGoogle Scholar
  16. 16.
    Pfeiffer, C. J., G. Rowden, and J. Weibel. 1974.Gastrointestinal Ultrastructure. Academic Press, Inc. New York, pp. 266–290.Google Scholar
  17. 17.
    Porter, K. R., D. Kelley, and P. M. Andrews. 1972. The preparation of cultured cells and soft tissues for scanning electron microscopy. In: Proc. Fifth Annual Stereoscan Colloquim. Kent Cambridge Scientific, Inc., Chicago, pp. 1–19.Google Scholar
  18. 18.
    Porter, K. R., and V. G. Fonte. 1973. Observations in the topography of normal and cancer cells. In:Scanning Electron Microscopy/1973. Part III. IITRI, Chicago, pp. 683–688.Google Scholar
  19. 19.
    Goldenberg, D. M., C. A. Pegram, and J. J. Vazquez. 1975. Identification of colon-specific antigen (CSA) in normal and neoplastic tissue. J. Immunol. 114: 1008–1013.PubMedGoogle Scholar
  20. 20.
    Bosmann, H. B., and T. C. Hall. 1974. Enzyme activity in invasive tumors of human breast and colon. Proc. Nat. Acad. Sci. USA 71: 1833–1837.PubMedCrossRefGoogle Scholar
  21. 21.
    Thomas, D. R., G. W. Philpott, and B. M. Jaffe. 1974. Prostaglandin E (PGE) control of cell proliferationin vitro: characteristics of HT-29. J. Surg. Res. 16: 463–465.PubMedCrossRefGoogle Scholar
  22. 22.
    Bloom, E. T., J. L. Fahey, I. A. Peterson, G. Geering, M. Bernhard, and G. Trempe. 1973. Anti-tumor activity in human serum: antibodies detecting blood-group-A-like antigen on the surface of tumor cells in culture. Br. J. Cancer 12: 21–31.Google Scholar
  23. 23.
    Rosai, J., T. W. Tillack, and V. T. Marchesi. 1972. Membrane antigens of human colonic carcinoma and non-tumoral colonic mucosa: results obtained with a new isolation method. Int. J. Cancer 10: 357–367.PubMedCrossRefGoogle Scholar
  24. 24.
    Morton, D. L. 1973. Horizons in tumor immunology. Surgery 74: 69–79.PubMedGoogle Scholar
  25. 25.
    Smith, R. T. 1972. Possibilities and problems of immunologic intervention in cancer. New Eng. J. Med. 287: 439–450.PubMedCrossRefGoogle Scholar
  26. 26.
    Kahan, B. D., B. H. Tom, K. K. Mittal, and J. J. Bergan. 1974. Immunodiagnostic test for transplant rejection. Lancet 1: 37–42.PubMedCrossRefGoogle Scholar
  27. 27.
    Tom, B. H., M. M. Jakstys, and B. D. Kahan. 1974. Leukocyte-aggregation: anin vitro assay for cell-mediated immunity. J. Immunol. 113: 1288–1295.PubMedGoogle Scholar
  28. 28.
    Tom, B. H., L. O. Huang, M. M. Jakstys, and B. D. Kahan. 1975. Characteristics of the leukocyte-aggregation assay for cell-mediated immunity. Clin. Exp. Immunol. 20: 131–141.PubMedGoogle Scholar
  29. 29.
    Rowley, J. D. 1973. Identification of a translocation with quinacrine fluorescence in a patient with acute leukemia. Ann. Genet. 16: 109–112.PubMedGoogle Scholar
  30. 30.
    Rowley, J. D. 1974. Do human tumors show a chromosome pattern specific for each etiologic agent? J. Natl. Cancer Inst. 52: 315–320.PubMedGoogle Scholar
  31. 31.
    Sandberg, A. A., and D. K. Hossfeld. 1970. Chromosomal abnormalities in human neoplasia. Ann. Rev. Med. 21: 379–408.PubMedCrossRefGoogle Scholar
  32. 32.
    Lubs, H. A. 1970. The clinical significance of chromosomal abnormalities in colonic cancer. In: W. J. Burdett (Ed.),Carcinoma of the colon and antecedent epithelium. Charles C. Thomas, Springfield, Illinois, pp. 319–332.Google Scholar
  33. 33.
    Cobb, L. M., and B. C. W. Mitchley. 1974. The growth of human tumors in immune deprived mice. Eur. J. Cancer 10: 473–476.PubMedGoogle Scholar
  34. 34.
    Goldenberg, D. M., R. D. Bhan, and R. A. Pavia. 1971.In vivo human-hamster somatic cell fusion indicated by glucose-6-phosphate dehydrogenase and lactate dehydrogenase profiles. Cancer Res. 31: 1148–1152.PubMedGoogle Scholar

Copyright information

© Tissue Culture Association 1976

Authors and Affiliations

  • Baldwin H. Tom
    • 1
    • 2
  • Lynne P. Rutzky
    • 1
    • 2
  • Milda M. Jakstys
    • 1
    • 2
  • Ryoichi Oyasu
    • 1
    • 2
  • Celia I. Kaye
    • 1
    • 2
  • Barry D. Kahan
    • 1
    • 2
  1. 1.Laboratory of Surgical Immunology of the Departments of Surgery and Physiology, Department of PathologyNorthwestern University Medical Center, Veterans Administration Lakeside HospitalChicago
  2. 2.Division of Genetics and Metabolism, Department of PediatricsCook County HospitalChicago

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