Biological Trace Element Research

, Volume 10, Issue 3, pp 215–222 | Cite as

Serum selenium concentrations in ovarian cancer patients using a simplified fluorimetric procedure

  • N. P. Das
  • C. W. Ma
  • Y. M. Salmon
Orginal Articles

Abstract

The serum selenium (Se) concentrations were determined to be 105.6±15.6 μg/L (\(\bar X \pm SD\)) and 116.7±18.4 μg/L (\(\bar X \pm SD\)) for the ovarian cancer patients and the control subjects in Singapore, respectively (p<0.0065). When we separated the patients into three age groups, namely <30, 30–50, and >50 yr, the ovarian cancer patients showed significantly lower mean serum Se levels than the control subjects for the 30–50-yr age group only. However, when the analysis of variance was used to evaluate the data, the values indicated that the age of the subject did not significantly affect the serum Se level. Our findings suggest that there is an inverse relationship between serum Se concentration and the incidence of human ovarian cancer.

A modified simple fluorimetric method for the determination of serum Se concentration is described. The procedure, with a sensitivity limit of 5 μg/L and percentage recoveries of 96.2–100.7%, requires only 0.2 mL of serum sample.

Index Entries

Serum selenium, concentrations in ovarian cancer patients fluorimetric method, for determination of serum Se ovarian cancer trace element carcinogenesis antioxidant nutritional anticancer agent 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    G. N. Schrauzer,Bioinorg. Chem. 5, 275 (1976).PubMedCrossRefGoogle Scholar
  2. 2.
    A. C. Griffin,Adv. Cancer Res. 29, 419 (1979).PubMedGoogle Scholar
  3. 3.
    G. N. Schrauzer, D. A. White, and C. J. Schneider,Bioinorg. Chem. 8, 387 (1978).PubMedCrossRefGoogle Scholar
  4. 4.
    C. Ip, and M. M. Ip,Carcinogenesis 2, 915 (1981).PubMedCrossRefGoogle Scholar
  5. 5.
    C. W. Welsch, M. Goodrich-Smith, C. K. Brown, H. D. Greene, and E. J. Hamel,Carcinogenesis 2, 519 (1981).PubMedCrossRefGoogle Scholar
  6. 6.
    R. J. Shamberger, and C. E. Willis,Crit. Rev. Clin. Lab. Sci. 2, 211 (1971).Google Scholar
  7. 7.
    G. N. Schrauzer, D. A. White, and C. J. Schneider,Bioinorg. Chem. 6, 265 (1977).CrossRefGoogle Scholar
  8. 8.
    G. N. Schrauzer, T. Molenaar, S. Mead, K. Kuehn, H. Yamamoto, and E. Araki,Jpn. J. Cancer Res. (Gann)76, 374 (1985).Google Scholar
  9. 9.
    K. P. McConnell, R. M. Jager, K. I. Bland, and C. G. Blotcky,J. Surg. Oncol. 15, 67 (1980).PubMedCrossRefGoogle Scholar
  10. 10.
    W. C. Willett, J. S. Morris, S. Pressel, J. O. Taylor, B. F. Polk, M. J. Stampfer, B. Rosner, K. Schneider, and C. G. Hames,Lancet ii,130 (1983).Google Scholar
  11. 11.
    Y. J. Chu, Q. Y. Liu, C. Hou, and S. Y. Yu,Biol. Trace Element Res. 6, 133 (1984).CrossRefGoogle Scholar
  12. 12.
    J. Aaseth, J. Alexander, Y. Thomassen, J. P. Blomboff, and S. Skrede,Clin. Biochem. 15, 281 (1982).PubMedCrossRefGoogle Scholar
  13. 13.
    J. A. Moore, R. Noiva, and I. C. Wells,Clin. Chem. 30, 1171 (1984).PubMedGoogle Scholar
  14. 14.
    O. Oster, W. Prellwitz, W. Kasper, and T. Meinertz,Clin. Chim. Acta 128, 125 (1983).PubMedCrossRefGoogle Scholar
  15. 15.
    P. Calautti, G. Moschini, B. M. Stievano, L. Tomio, F. Calzavara, and G. Perona,Scand. J. Haematol. 24, 63 (1980).PubMedCrossRefGoogle Scholar
  16. 16.
    J. H. Watkinson,Anal. Chem. 38, 92 (1966).PubMedCrossRefGoogle Scholar
  17. 17.
    G. Alfthan,Anal. Chim. Acta 165, 187 (1984).CrossRefGoogle Scholar
  18. 18.
    J. E. Spallholz, G. F. Collins, and K. Schwarz,Bioinorg. Chem. 9, 453 (1978).CrossRefGoogle Scholar
  19. 19.
    H. Sundstrom, H. Korpela, L. Viinikka, and A. Kauppila,Cancer Lett. 24, 1 (1984).PubMedCrossRefGoogle Scholar
  20. 20.
    J. T. Rotruck, A. L. Pope, H. E. Ganther, D. G. Hafeman, A. B. Swanson, and W. G. Hoekstra,Science 179, 588 (1973).PubMedCrossRefGoogle Scholar
  21. 21.
    R. A. Sunde and W. G. Hoekstra,Nutr. Rev. 38, 265 (1980).PubMedCrossRefGoogle Scholar
  22. 22.
    G. N. Schrauzer (ed.), inVitamins, Nutrition, and Cancer, Prasad, Karger, Basel, 1984, pp. 240–250.Google Scholar
  23. 23.
    G. N. Schrauzer and D. A. White,Bioinorg. Chem. 8, 303 (1978).PubMedCrossRefGoogle Scholar
  24. 24.
    L. Lalonde, Y. Jean, K. D. Roberts, A. Chapdelaine, and G. Bleau,Clin. Chem. 28, 172 (1982).PubMedGoogle Scholar
  25. 25.
    G. N. Schrauzer, W. J. Rhead, and G. A. Evans,Bioinorg. Chem. 2, 329 (1973).CrossRefGoogle Scholar
  26. 26.
    J. T. Salonen, R. Salonen, R. Lappetelainen, P. H. Maenpaa, G. Alfthan, and P. Puska,Br. Med. J. 290, 417 (1985).Google Scholar

Copyright information

© The Humana Press Inc 1986

Authors and Affiliations

  • N. P. Das
    • 1
  • C. W. Ma
    • 1
  • Y. M. Salmon
    • 2
  1. 1.Department of Biochemistry, Faculty of MedicineNational University of SingaporeSingapore
  2. 2.‘B’ UnitKandang Kerbau HospitalSingapore

Personalised recommendations