Biological Trace Element Research

, Volume 37, Issue 1, pp 39–50 | Cite as

Nickel chloride inhibits the DNA repair of UV-treated but not methyl methanesulfonate-treated chinese hamster ovary cells

  • S. F. Lee-Chen
  • M. C. Wang
  • C. T. Yu
  • D. R. Wu
  • K. Y. Jan
Article

Abstract

Nickel, a human carcinogen, has been shown to enhance the cytotoxicity, mutagenicity, and sister-chromatid exchanges (SCE) induced by ultraviolet (UV) light but not by methyl methanesulfonate (MMS). To verify that the cocytotoxicity and cogenotoxicity of nickel are correlated with its inhibition on DNA repair, the effects of nickel on the DNA repair induced by UV and by MMS have been investigated. Our analyses of DNA repair of single-strand breaks by alkaline elution and alkaline sucrose sedimentation indicate that nickel inhibited the DNA repair in UV-treated, but not in MMS-treated cells. Therefore, the inhibition of DNA repair seems to play an important role in the cocytotoxicity and comutagenicity of nickel. However, the inhibition of DNA repair seems not to play a decisive role in enhancing SCE, because we have previously shown that arsenite inhibits the UV-induced DNA repair, but has no enhancing effect on the UV-induced SCE. Our results also show that nickel had obvious inhibitory effects on DNA ligation and postreplication repair, but had no apparent effect on nucleotide excision and DNA polymerization in the UV repair. The results of the DNA ligation inhibition by nickel in UV but not in MMS repair suggest that different ligases are used in the DNA repair of UV- and MMS-induced damages.

Index Entries

DNA repair nickel metal ultraviolet light methyl methanesulfonate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    F. W. Sunderman, Jr., inEnvironmental Carcinogens—Selected Methods of Analysis, International Agency for Research on Cancer, vol. 8., I. K. O'Neill, P. Schuller and L. Fishbein, eds., Lyon, 1986, pp. 17–43.Google Scholar
  2. 2.
    IARC inIARC Monogr. Evalu. Carcinog. Risk of Chemicals to Man. IARC, vol. 11, Lyon, 1976, pp. 75–112.Google Scholar
  3. 3.
    P. Sen and M. Costa,Cancer Res. 45, 2320 (1985).PubMedGoogle Scholar
  4. 4.
    K. Conway, X.-W. Wang, L.-S. Xu, and M. Costa,Carcinogenesis 8, 1115 (1987).PubMedCrossRefGoogle Scholar
  5. 5.
    E. Gebhart and T. G. Rossman, inMetals and Their Compounds in the Environment, E. Merian, ed., VCH Verlagsgesellshaft, Weinheim, GDR, 1989, pp. 617–640.Google Scholar
  6. 6.
    A. Hartwig and D. Beyersmann,Mutat. Res. 217, 65 (1989).PubMedGoogle Scholar
  7. 7.
    R. D. Snyder, G. F. Davis, and P. J. Lachmann,Biol. Trace Element Res. 21, 389 (1989).Google Scholar
  8. 8.
    J. S. Dubins and J. M. LaVelle,Mutat. Res. 162, 187 (1986).PubMedGoogle Scholar
  9. 9.
    K. Y. Jan, Y. C. Lin, I. C. Ho, S. L. Kao, and T. C. Lee,toxicol. Lett. 51, 81 (1990).PubMedCrossRefGoogle Scholar
  10. 10.
    K. W. Kohn, R. A. G. Ewig, C. Erickson, and I. A. Zwelling, inDNA Repair: A Laboratory Manual of Research Procedures, vol. I, Part B, E. C. Friedberg and P. C. Hanawalt, eds., Marcel Dekker, New York, 1981, pp. 379–401.Google Scholar
  11. 11.
    S. F. Lee-Chen, C. T. Yu, and K. Y. Jan,Mutagenesis 7, 51 (1991).CrossRefGoogle Scholar
  12. 12.
    K. Yamada, F. Hanoka, and M. Yamada,J. Biol. Chem. 260, 10,412 (1985).Google Scholar
  13. 13.
    M. Cordeiro-Stone, J. C. Boyer, B. A. Smith, and W. K. Kaufmann,Carcinogenesis 7, 1775 (1986).PubMedCrossRefGoogle Scholar
  14. 14.
    B. Palcic and L. D. Skarsgard,Int. J. Radiat. Biol. 21, 417 (1972).CrossRefGoogle Scholar
  15. 15.
    C. M. Gredik and A. R. Collins,Mutat. Res. 254, 231 (1991).Google Scholar
  16. 16.
    T. C. Lee, R. Y. Huang, and K. Y. Jan,Mutat. Res. 148, 83 (1985).PubMedGoogle Scholar
  17. 17.
    R. K. Sahu, S. P. Katsifis, P. L. Kinney, and N. T. Christie,Mol. Toxicol. 2, 129 (1989).PubMedGoogle Scholar
  18. 18.
    K. Erixon, inMechanism of DNA Damage and Repair, M. G. Simic, L. Grossman and A.D. Upton, eds., Plenum, New York, 1986, pp. 159–170.Google Scholar
  19. 19.
    N. T. Christie and D. M. Tummolo,Biol. Trace Element Res. 21, 3 (1989).CrossRefGoogle Scholar
  20. 20.
    G. Ahnstrom and M. Ljungman,Mutat. Res. 194, 17 (1988).PubMedGoogle Scholar
  21. 21.
    F. Otsuka, H. Ohno, T. Enomoto, F. Hanaoka, M. Ohsawa, and M. Yamada,Mutat. Res. 161, 75 (1986).PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • S. F. Lee-Chen
    • 1
  • M. C. Wang
    • 1
  • C. T. Yu
    • 1
  • D. R. Wu
    • 1
  • K. Y. Jan
    • 1
  1. 1.Institute of ZoologyAcademia SinicaTaipeiRepublic of China

Personalised recommendations