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Molecular Biotechnology

, Volume 5, Issue 2, pp 97–99 | Cite as

Isolation and characterization of the restriction endonucleasePpel fromPhormidium persicinum

  • Stanislaw Piechula
  • Jacek Piosik
  • Krzysztof Bielawski
  • Anna J. Podhajska
Research Research Note

Abstract

PpeI is a type II restriction endonuclease isolated from cyanobacterial strainPhormidium persicinum. The endonuclease PpeI, an isoschizomer of ApaI, recognizes the hexanucleotide sequence (5’t-GGGCC/C-3’t) and cleaves, after the second C, producing four nucleotide 3’t-cohesive ends.

Index entries

Phormidium persicinum cyanobacteria restriction endonuclease isoschizomer cleavage position palindrome ApaI endonuclease 

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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Stanislaw Piechula
    • 1
  • Jacek Piosik
    • 1
  • Krzysztof Bielawski
    • 1
  • Anna J. Podhajska
    • 1
  1. 1.Department of MicrobiologyUniversity of GdanskKładki 24Poland

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