Pretreatment of corn fiber by pressure cooking in water
The pretreatment of corn fiber using liquid water at temperatures between 220 and 260°C enhances enzymatic hydrolysis. This paper describes the laboratory reactor system currently in use for cooking of corn fiber at temperatures ranging from 200 to 260°C. The corn fiber at approx 4.4% solid/liquid slurry was treated in a 2-L, 304 SS, Parr reactor with three turbine propeller agitators and a Proportional-Integral-Derivative (PID), controller that controlled temperature within ±1°C. Heat-up times to the final temperatures of 220, 240, or 260°C were achieved in 50 to 60 min. Hold time at the final temperature was less than 10 s. A serpentine cooling coil, through which tap water was circulated at the completion of the run, cooled the reactor’s contents to 180°C within 2 min after the maximum temperature was attained. Ports in the reactor’s head plate facilitated sampling of the slurry and monitoring the pH. A continuous pH monitoring system was developed to help observe trends in pH during pretreatment and to assist in the development of a base (2.0M KOH) addition profile to help keep the pH within the range of 5.0 to 7.0. Enzymatic hydrolysis gave 33 to 84% conversion of cellulose in the pretreated fiber to glucose compared to 17% for untreated fiber.
Index EntriesCorn fiber water pretreatment enzyme hydrolysis cellulose glucose
Unable to display preview. Download preview PDF.
- 2.Haw, J. F., Maciel, G. E., Linden, J. C., and Murphy, V. G. (1985),Holzforschung 39, 99–107.Google Scholar
- 7.Foody, P. (July 24,1984), US patent # 4,461,648.Google Scholar
- 11.Ladisch, M. R., Waugh, L., Westgate, P., Kohlmann, K., Hendrickson, R., Yang, Y., and Ladisch, C. (1992), in Ladisch, M. R. and Bose, A., eds., American Chemical Society, Washington, DC, 510-518.Google Scholar
- 12.Kohlmann, K. L., Westgate, P. J., Weil, J., and Ladisch, M. R. (1994),J. Aerospace 102, 1476–1483.Google Scholar
- 14.Kohlmann, K. L., Sarikaya, A., Westgate, P. J., Weil, J., Velayudhan, A., Hendrickson, R., and Ladisch, M. R. (1995), in Penner, M. and Saddler, J., eds., American Chemical Society, Washington, DC, No. 618, 237-255.Google Scholar
- 16.NREL, Chemical Analysis and Testing Standard Procedures, Golden, CO (1994).Google Scholar