Safe disposal of diisopropyl fluorophosphate (DFP)
Article
Received:
Accepted:
Abstract
Diisopropyl fluorophosphate (DFP), a volatile highly toxic enzyme inhibitor, in buffer (pH 3, pH 5, pH 7, pH 9, pH 11, Hank’s, Dulbecco’s, PBS, TBE, and HEPES) or water (10 mM), in DMF solution (200 mM), and bulk quantities can be degraded by adding 1M NaOH. The DFP was completely degraded, as determined by enzymatic assay, and the final reaction mixtures were not mutagenic.
Index entries
Safety degradation stability enzyme inhibitor diisopropyl fluorophosphate dyflos IsoflurophatePreview
Unable to display preview. Download preview PDF.
References
- 1.Cohen, J. A., Oosterbaan, R. A., and Berends, F. (1967), inMethods in Enzymology, vol. 11, Hirs, C. H. W., ed., Academic, New York, pp. 686–702.Google Scholar
- 2.Saunders, B. C. and Stacey, G. J. (1948),J. Chem. Soc, 695–699.Google Scholar
- 3.Caughey, G. H., Viro, N. F., Lazarus, S. C, and Nadel, J. A. (1988),Biochim. Biophys. Acta 952, 142–149.Google Scholar
- 4.Park, J. H., Lee, Y. S., Chung, C. H., and Goldberg, A. L. (1988),J. Bacteriol. 170, 921–926.Google Scholar
- 5.Zanglis, A. and Lianos, E. A. (1987),J. Lab. Clin. Med. 110, 330–337.Google Scholar
- 6.Fried, V. A., Smith, H. T., Hildebrandt, E., and Weiner, K. (1987),Proc. Natl. Acad. Sci. USA 84, 3685–3689.CrossRefGoogle Scholar
- 7.Schwartz, L. B. and Bradford, T. R. (1986),J. Biol. Chem. 261, 7372–7379.Google Scholar
- 8.Cohen, M. L., Geary, L. E., and Wiley, K. S. (1983),J. Pharmacol. Exp. Ther. 224, 379–385.Google Scholar
- 9.Arky, R., ed. (1994),Physicians’ Desk Reference, 48th ed., Medical Economics Data, Montvale, NJ, pp. 1457,1458.Google Scholar
- 10.Wilson, B. W. and Walker, C. R. (1974),Proc. Natl. Acad. Sci. USA 71, 3194–3198.CrossRefGoogle Scholar
- 11.Gordon, C. J. and MacPhail, R. C. (1993),J. Toxicol. Envir. Health 38, 257–271.CrossRefGoogle Scholar
- 12.Epstein, J. (1970),Science 170, 1396–1398.CrossRefGoogle Scholar
- 13.Epstein, J. (1974),J. Am. Water Works Assoc. 66, 31–37.Google Scholar
- 14.Small, M. J. (1984),Compounds Formed from the Chemical Decontamination of HD, GB, and VX and Their Environmental Fate (USAMBRDL-TR-8304; ADA 149515), US Army Medical Research and Development Command, Fort Detrick, Frederick, MD.Google Scholar
- 15.Yang, Y.C., Baker, J. A., and Ward, J. R. (1992),Chan. Rev. 92, 1729–1743.Google Scholar
- 16.Ryan, J. A., McGaughran, W. R., Lindemann, C. J., and Zacchei, A. G. (1979),J. Pharm. Sci. 68, 1194,1195.CrossRefGoogle Scholar
- 17.Anon. (1986),Diisopropyl Fluorophosphate, Technical Information Bulletin Number AL-122, Aldrich Chemical Co., Milwaukee, WI.Google Scholar
- 18.Hummel, B. C. W. (1959),Can. J. Biochem. Physiol. 37, 1393–1399.Google Scholar
- 19.Rao, K. N. and Lombardi, B. (1975),Anal. Biochem. 65, 548–551.CrossRefGoogle Scholar
- 20.Lunn, G. and Sansone, E. B. (1989),J. Chem. Educ. 66, 443–445.CrossRefGoogle Scholar
- 21.Lunn, G., Sansone, E. B., and Andrews, A. W. (1991),Environ. Molec. Mutagen. 17, 59–62.CrossRefGoogle Scholar
- 22.Beyer, W. H., ed. (1968),Handbook of Tables for Probability and Statistics, 2nd ed., Chemical Rubber Co., Cleveland, OH, pp. 339–345.Google Scholar
- 23.Ellin, R. I., Groff, W. A., and Kaminskis, A. (1981),J. Environ. Sci. Health B16, 713–717.Google Scholar
Copyright information
© Humana Press Inc 1994