Applied Biochemistry and Biotechnology

, Volume 50, Issue 1, pp 35–43 | Cite as

Chemical synthesis and cloning of human Β-endorphin gene inEscherichia coli

  • Myoung Hee Kim
  • Hyun Ju Lee
  • Kyung T. Min
Article
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Accepted:

Abstract

Total synthesis of human Β-endorphin gene has been designed for the expression in bacterial system. Eight individual oligonucleotides corresponding to the Β-endorphin gene were chemically synthesized and joined through the enzyme-catalyzed reaction. The final yield of the 111-nucleotide-long synthetic Β-endorphin gene construct was about 10% of the total oligonucleotide used. The synthetic human Β-endorphin gene was cloned into the bacteriumEscherichia coli, using pUC8 vector and shown to have the correct nucleotide sequences as designed.

Index Entries

Β-endorphin human gene oligonucleotide synthesis recombinant DNA Escherichia coli 
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Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Myoung Hee Kim
    • 1
  • Hyun Ju Lee
    • 1
  • Kyung T. Min
    • 1
  1. 1.Genetic Resources CenterGenetic Engineering Research Institute, KISTTaejonKorea

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