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Applied Biochemistry and Biotechnology

, Volume 67, Issue 1–2, pp 139–152 | Cite as

Effect of light irradiance on the production of sulfolipids from Anabaena 7120 in a fed-batch photobioreactor

  • Shivaun D. Archer
  • Karen A. McDonald
  • Alan P. Jackman
Article

Abstract

Sulfolipids have recently emerged as promising antiHIV and antitumor therapeutics. These lipids have been found in association with the photosynthetic apparatus in most photoautotrophic organisms. To date there have been no quantitative studies on the effect of environmental factors on the production of sulfolipid. In this study, we present results on the effect of light irradiance on the production of sulfolipids using the cyanobacteriumAnabaena 7120. The cyanobacteria are grown in a 2 L fedbatch photobioreactor at various external-light intensities. Total lipids are extracted using the Folsch procedure and sulfolipids are quantified using thin-layer chromatography and scanning densitometry. We have achieved a maximum of 14 mg sulfolipid/g dry weight of cell. Our results indicate that there are two stages in the specific rate of production of sulfolipids, one in the declining exponential-growth phase of the cells and the other in the light-limited stage of growth.

Index Entries

Sulfolipid light intensity fed-batch culture cyano-bacteria photobioreactor 

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Copyright information

© Humana Press Inc. 1997

Authors and Affiliations

  • Shivaun D. Archer
    • 1
  • Karen A. McDonald
    • 1
  • Alan P. Jackman
    • 1
  1. 1.Department of Chemical Engineering and Materials ScienceUniversity of CaliforniaDavis

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