Applied Biochemistry and Biotechnology

, Volume 59, Issue 2, pp 167–175 | Cite as

Selectivity of methyl-fructoside synthesis with Β-fructofuranosidase

  • Maria Rodríguez
  • Alfonso Gómez
  • Fernando González
  • Eduardo Barzana
  • Agustin López-Munguía
Original Articles

Abstract

Enzyme synthesis of methyl fructoside was studied usingΒ-fructofuranosidase fromSacharomyces cerevisiae and sucrose and methanol as substrates. Taking into account the inhibition and deactivation effects of methanol on the enzyme, a system with 4.9M (20%, v/v) methanol was selected. At this alcohol level, 35% of sucrose is converted to fructoside at low or high substrate concentrations. The effect of enzyme concentration, pH, and temperature on both the synthesis and the hydrolysis of the fructoside was investigated. It was found that if the reaction proceeds at pH 6.0, 4‡C and/or 0.014 mg/mL (3 U/mL) of Β-fructofuranosidase at varying sucrose concentrations, methyl fructoside may be obtained with a minimum loss of the fructoside at the end of the reaction.

Index Entries

Alcoholysis Methyl-fructoside Β-fructofuranosidase invertase 

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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Maria Rodríguez
    • 1
  • Alfonso Gómez
    • 1
  • Fernando González
    • 1
  • Eduardo Barzana
    • 2
  • Agustin López-Munguía
    • 1
    • 2
  1. 1.Institute de BiotecnologiaUNAMMorelosMéxico
  2. 2.Facultad de QuimicaUNAM, Cd, UniversitariaD.F.México

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