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Applied Biochemistry and Biotechnology

, Volume 75, Issue 2–3, pp 279–293 | Cite as

Interaction between indigo and adsorbed protein as a major factor causing backstaining during cellulase treatment of cotton fabrics

  • Alexander V. Gusakov
  • Arkady P. Sinitsyn
  • Alejandro G. Berlin
  • Nonna N. Popova
  • Alexander V. Markov
  • Oleg N. Okunev
  • Dmitry F. Tikhomirov
  • Mark Emalfarb
Original Ariticle

Abstract

A model microassay system was developed to measure indigo backstaining on cotton fabrics in the presence of enzymes on a small laboratory scale. Backstaining indexes for 11 cellulase samples were measured, and the enzymes were ranked from lower to higher backstaining. Two multienzyme cellulase preparations were separated into fractions using chromatofocusing on a Mono P column. Adsorption ability and backstaining properties of purified enzyme fractions were studied. Evidence was obtained that protein adsorption on cotton fabrics is a crucial parameter causing backstaining (both for crude cellulase samples and purified enzyme components).

Index entries

Cellulase adsorption textile denim stone washing indigo backstaining microassay 

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References

  1. 1.
    Klahorst, S., Kumar, A., and Mullins, M. M. (1994),Textile Chemist Colorist 26, 13–18.Google Scholar
  2. 2.
    Koo, H., Ueda, M., Wakida, T., Yoshimura, Y., and Igarashi, T. (1994),Textile Res. J. 64, 70–74.CrossRefGoogle Scholar
  3. 3.
    Kumar, A., Yoon, M.-Y., and Purtell, C. (1997),Textile Chemist Colorist 29, 37–42.Google Scholar
  4. 4.
    Cavaco-Paulo, A., Morgado, J., Almeida, L., and Kilburn, D. (1998),Textile Res. J.,68, 398–401.Google Scholar
  5. 5.
    Ghose, T. K. (1987),Pure Appl. Chem. 59, 257–268.CrossRefGoogle Scholar
  6. 6.
    Klyosov, A. A. and Rabinowitch, M. L. (1980), inEnzyme Engineering—Future Directions (Wingard, L. B., ed.), Plenum, New York, pp. 83–165.Google Scholar
  7. 7.
    Sinitsyn, A. P., Chernoglazov, V. M., and Gusakov, A. V. (1990),Methods of Investigation and Properties of Cellulolytic Enzymes, Biotechnology Series, vol. 25, VINITI, Moscow, Russia.Google Scholar
  8. 8.
    Wood, T. M. and Bhat, K. M. (1988),Methods Enzymol. 160, 87–112.CrossRefGoogle Scholar
  9. 9.
    Dawson, R. M. C., Elliott, D. C., Elliott, W. H., and Jones, K. M. (1986),Data for Biochemical Research, 3rd ed., Clarendon Press, Oxford.Google Scholar

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Alexander V. Gusakov
    • 1
  • Arkady P. Sinitsyn
    • 1
  • Alejandro G. Berlin
    • 1
  • Nonna N. Popova
    • 1
  • Alexander V. Markov
    • 1
  • Oleg N. Okunev
    • 2
  • Dmitry F. Tikhomirov
    • 3
  • Mark Emalfarb
    • 3
  1. 1.Department of ChemistryM. V. Lomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute of Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesPushchinoRussia
  3. 3.AARL, Inc.Jupiter

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