Advertisement

Applied Biochemistry and Biotechnology

, Volume 56, Issue 2, pp 169–180 | Cite as

Biotransformation from geraniol to nerol by immobilized grapevine cells (V. vinifera)

  • J. Guardiola
  • J. L. Iborra
  • L. Ródenas
  • M. Cánovas
Original Articles

Abstract

The abilities of two grapevine cell suspensions (Vitis vinifera L. cv. Gamay Fréaux andVitis vinifera L. cv. Monastrell) to biotransform geraniol into nerol in a biphasic system based on the culture medium and Miglyol 812 were compared. The Gamay grape cell suspension was able to transform higher concentrations of geraniol into nerol than the Monastrell one. Gamay grape cells were immobilized in both calcium alginate beads and polyurethane foams. The cytotoxic effect of increasing concentrations of geraniol, as well as the ability of the immobilized cells to biotransform geraniol into nerol, was checked. Immobilization proved to be advantageous in protecting cells against the toxicity of the substrate. Furthermore, immobilization also seemed to have an effect on the secondary metabolism, the cells immobilized in polyurethane foams being more efficient at performing the isomerization process (40% conversion of geraniol into nerol) than both the freely suspended and calcium alginate immobilized cells (20% conversion).

Index Entries

Immobilized plant cells biotransformations Vitis vinifera monoterpenes biphasic systems cell viability 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Morris, P., Scragg, A. H., Smart, N. J., and Stafford, A. (1985), inPlant Cell Culture. A Practical Approach, Dixon, R. A., ed., IRL Press, Oxford, pp. 127–168.Google Scholar
  2. 2.
    Williams, P. D. and Mavituna, F. (1992), inPlant Biotechnology, Comprehensive Biotechnology Second Supplement, Fowler, M. W. and Waurer, G. S., eds., Pergamon, Oxford, pp. 63–78.Google Scholar
  3. 3.
    Dervakos, G. A. and Webb, C. (1991),Biotechnol. Adv. 9, 559.CrossRefGoogle Scholar
  4. 4.
    Mulder-Krieger, TH., Verpoorte, R., Baerheim Svendsen, A., and Scheffer, J. J. C. (1988),Plant Cell Tissue Org. Cult. 13, 85.CrossRefGoogle Scholar
  5. 5.
    Brown, J. T., Hegarty, P. K., and Charlwood, B. V. (1987),Plant Sci. 48, 195.CrossRefGoogle Scholar
  6. 6.
    Berlin, J., Witte, L., Schubert, W., and Wray, V. (1984),Phytochemistry 23, 1277.CrossRefGoogle Scholar
  7. 7.
    Mavituna, F. and Park, J. M. (1985),Biotechnol. Lett. 7, 637.CrossRefGoogle Scholar
  8. 8.
    Iborra, J. L., Guardiola, J., Montaner, S., Cánovas, M., and Manjón, A. (1992),Biotechnol. Tech. 6, 319.CrossRefGoogle Scholar
  9. 9.
    Ambid, C., Moisseeff, M., and Fallot, J. (1982),Plant Cell Rep. 1, 91.CrossRefGoogle Scholar
  10. 10.
    Ambid, C., Moisseeff, M., and Fallot, J. (1983),Physiol. Vég. 21, 87.Google Scholar
  11. 11.
    Carriere, F., Gil, G., Tapie, P., and Chagvardieff, P. (1989),Phytochemistry 28, 1087.CrossRefGoogle Scholar
  12. 12.
    Gbolade, A. A. and Lockwood, G. B. (1989),Z. Naturforsch. Sect. C. Biosci. 44, 1066.Google Scholar
  13. 13.
    Croteau, R. and Winters, J. N. (1982),Plant Physiol. 69, 975.CrossRefGoogle Scholar
  14. 14.
    Croteau, R. and Sood, V. K. (1985),Plant Physiol. 77, 801.Google Scholar
  15. 15.
    Paisarnrat, S. (1985),Métabolisation des Alcools Monoterpéniques par des Cellules de Raisin Muscat Cultivées in vitro, Doctoral thesis, Institut National Polytechnique de Toulouse, France, p. 156.Google Scholar
  16. 16.
    Cormier, F. and Ambid, C. (1987),Plant Cell Rep. 6, 427.Google Scholar
  17. 17.
    Barros, M. R. Aires, Cabral, J. M. S., and Novais, J. M. (1987),Biotechnol. Bioeng. 29, 1097.CrossRefGoogle Scholar
  18. 18.
    Schueler, C., Van der Meer, A. B., Van Lelyveld, P. H., and Joosten, G. E. H. (1984),Prog. Ind. Microbiol. 20, 85.Google Scholar
  19. 19.
    Deno, H., Suga, C., Morimoto, T., and Fujita, Y. (1987),Plant Cell Rep. 6, 197.CrossRefGoogle Scholar
  20. 20.
    Brodelius, P., Deus, B., Mosbach, K., and Zenk, M. H. (1979),FEBS Lett. 103, 93.CrossRefGoogle Scholar
  21. 21.
    Vanek, T., Urmantseva, V. V., Wimmmer, Z., and Macek, T. (1989),Biotechnol. Lett. 11, 243.CrossRefGoogle Scholar
  22. 22.
    Corchete, P. and Yeoman, M. M. (1989),Plant Cell Rep. 8, 128.CrossRefGoogle Scholar
  23. 23.
    Gbolade, A. A. and Lockwood, G. B. (1990),Z. Naturforsch. Sect. C. Biosci. 45, 245.Google Scholar

Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • J. Guardiola
    • 1
  • J. L. Iborra
    • 1
  • L. Ródenas
    • 1
  • M. Cánovas
    • 1
  1. 1.Department of Biochemistry and Molecular Biology B and Immunology, Faculty of ChemistryUniversity of MurciaMurciaSpain

Personalised recommendations