Applied Biochemistry and Biotechnology

, Volume 61, Issue 1–2, pp 189–198 | Cite as

Cyclodextrins as hosts for poorly water-soluble compounds in enzyme catalysis

  • Roque Bru
  • Jose M. López-Nicolás
  • Estrella Núñez-Delicado
  • Dolores Nortes-Ruipérez
  • Alvaro Sánchez-Ferrer
  • Francisco Garciá-Carmona


The capability of cyclodextrins to enhance greatly the solubility in water of poorly water-soluble substances makes them an ideal alter native for investigating the expression of enzyme activity with such substrates in aqueous solution. This capability is demonstrated by using soybean lipoxygenase with linoleic acid/β-cyclodextrin and di ethylstilbestrol/γ-cyclodextrin, and cholesterol oxidase with choles terol/methyl-β-cyclodextrin.

Index entries

Cyclodextrins linoleic acid diethylstilbestrol cholesterol lipoxygenase cholesterol oxidase 















linoleic acid


critical micelle concentration




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Copyright information

© Humana Press Inc 1997

Authors and Affiliations

  • Roque Bru
    • 1
  • Jose M. López-Nicolás
    • 1
  • Estrella Núñez-Delicado
    • 1
  • Dolores Nortes-Ruipérez
    • 1
  • Alvaro Sánchez-Ferrer
    • 1
  • Francisco Garciá-Carmona
    • 1
  1. 1.Departamento de Bioquimica y Biologia Molecular A, Facultad de BiologiaUniversidad de MurciaMurciaSpain

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