Applied Biochemistry and Biotechnology

, Volume 61, Issue 1–2, pp 189–198 | Cite as

Cyclodextrins as hosts for poorly water-soluble compounds in enzyme catalysis

  • Roque Bru
  • Jose M. López-Nicolás
  • Estrella Núñez-Delicado
  • Dolores Nortes-Ruipérez
  • Alvaro Sánchez-Ferrer
  • Francisco Garciá-Carmona
Article

Abstract

The capability of cyclodextrins to enhance greatly the solubility in water of poorly water-soluble substances makes them an ideal alter native for investigating the expression of enzyme activity with such substrates in aqueous solution. This capability is demonstrated by using soybean lipoxygenase with linoleic acid/β-cyclodextrin and di ethylstilbestrol/γ-cyclodextrin, and cholesterol oxidase with choles terol/methyl-β-cyclodextrin.

Index entries

Cyclodextrins linoleic acid diethylstilbestrol cholesterol lipoxygenase cholesterol oxidase 

Abbreviations

α-CD

alpha-cyclodextrin

β-CD

beta-cyclodextrin

mβ-CD

methyl-beta-cyclodextrin

hpβ-CD

2-hydroxypropylpropyl-beta-cyclodextrin

malβ-CD

maltosyl-beta-cyclodextrin

γCD

gamma-cyclodextrin

LA

linoleic acid

CMC

critical micelle concentration

DES

diethylstilbestrol

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Copyright information

© Humana Press Inc 1997

Authors and Affiliations

  • Roque Bru
    • 1
  • Jose M. López-Nicolás
    • 1
  • Estrella Núñez-Delicado
    • 1
  • Dolores Nortes-Ruipérez
    • 1
  • Alvaro Sánchez-Ferrer
    • 1
  • Francisco Garciá-Carmona
    • 1
  1. 1.Departamento de Bioquimica y Biologia Molecular A, Facultad de BiologiaUniversidad de MurciaMurciaSpain

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