Biological Trace Element Research

, Volume 53, Issue 1–3, pp 65–83 | Cite as

Selenium-vitamin E supplementation in infertile men

Effects on semen parameters and micronutrient levels and distribution
  • Denis Vézina
  • François Mauffette
  • Kenneth D. Roberts
  • Gilles Bleau
Original Articles

Abstract

In order to verify the hypothesis that selenium (Se) and vitamin E (Vit E) could improve male fertility, nine oligoasthenoteratozoospermic men were supplemented for a period of 6 mo with Se and Vit E. Compared to the baseline period (presupplementation) of 4 mo, statistically significant increases were observed for Se and Vit E levels, sperm motility, percent live, and percent normal spermatozoa. These improvements are likely to be “supplementation-dependent,” since all of the parameters returned to baseline values during the posttreatment period. None of the couples reported a pregnancy during the study. The HPLC analysis conducted on the serum of one of the patients showed the existence of at least six different Se-containing peaks, whose Se content was affected by supplementation. The mechanism(s) involved in these improvements of semen parameters is presently under investigation.

Index Entries

Selenium vitamin E infertility 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    G. Bleau, J. Lemarbre, G. Faucher, K. D. Roberts, and A. Chapdelaline, Semen selenium and human fertility,Fertil. Steril. 42, 890–894 (1984).PubMedGoogle Scholar
  2. 2.
    D. Behne, H. Gebner, G. Wolters and J. Brotherton, Selenium, rubidium and zinc in human semen and semen fractions,Int. J. Androl. 11, 415–423 (1988).PubMedGoogle Scholar
  3. 3.
    G. Noack-Füller, C. De Beer and H. Sweibert, Cadmium, lead, selenium, and zinc in semen of occupationally unexposed men,Andrologia 25, 7–12 (1993).PubMedCrossRefGoogle Scholar
  4. 4.
    B. Xu, S. E. Chia, M. Tsakok and C. N. Ong, Trace elements in blood and seminal plasma and their relationship to sperm quality,Reprod. Toxicol. 7, 613–618 (1993).PubMedCrossRefGoogle Scholar
  5. 5.
    M. Saaranen, U. Suistomaa, M. Kantola, S. Saarikoski and T. Vanha-Perttula, Lead, magnesium, selenium and zinc in human seminal fluid: comparison with semen parameters and fertility,Hum. Reprod. 2, 475–479 (1987).PubMedGoogle Scholar
  6. 6.
    A. C. Roy, R. Karunanithy and S. S. Ratnam, Lack of correlation of selenium level in human semen with sperm count/motility,Arch. Androl. 25, 59–62 (1990).PubMedCrossRefGoogle Scholar
  7. 7.
    H. I. Calvin and G. W. Cooper, A specific selenopolypeptide associated with the outer membrane of rat sperm mitochondria, inThe Spermatozoon, D. W. Fawcett and J. M. Bedford, eds., Urban and Schwarzenberg, Baltimore, pp. 464–467 (1979).Google Scholar
  8. 8.
    H. I. Calvin, E. Wallace, and G. W. Cooper, The role of selenium in the organization of the mitochondrial helix in rodent spermatozoa, inSelenium in Biology and Medicine, J. E. Spallholz, J. L. Martin, and H. E. Ganther, eds., AVI Publishing Company, Westport, pp. 319–324 (1981).Google Scholar
  9. 9.
    K. P. McConnell and R. M. Burton, Selenium and spermatogenesis, inSelenium in Biology and Medicine, J. E. Spallholz, J. L. Martin, and H. E. Ganther, eds., AVI Publishing Company, Westport, pp. 132–145 (1981).Google Scholar
  10. 10.
    E. Wallace, G. W. Cooper, and H. I. Calvin, Effects of selenium deficiency on the shape and arrangement of rodent sperm mitochondria,Gamete Res. 4, 389–399 (1983).CrossRefGoogle Scholar
  11. 11.
    E. Wallace, H. I. Calvin, K. Ploetz, and G. W. Cooper, Functional and developmental studies on the role of selenium in spermatogenesis, inSelenium in Biology and Medicine, part A, G. F. Combs, Jr., J. E. Spallholz, O. A. Levander, and J. E. Oldfield, eds., AVI, New York, pp. 181–196 (1987).Google Scholar
  12. 12.
    T. Watanabe and A. Endo, Effects of selenium deficiency on sperm morphology and spermatocyte chromosomes in mice,Mutation Res. 262, 93–99 (1991).PubMedCrossRefGoogle Scholar
  13. 13.
    A. Roveri, A. Casasco, M. Maiorino, P. Dalan, A. Calligaro and F. Ursini, Phospholipid hydroperoxide glutathione peroxidase of rat testis: gonadotropin dependence and immunocytochemical identification,J. Biol. Chem. 267, 6142–6146 (1992).PubMedGoogle Scholar
  14. 14.
    J. G. Alvarez and B. T. Storey, Lipid peroxidation and the reactions of superoxide and hydrogen peroxide in mouse spermatozoa,Biol. Reprod. 30, 833–841 (1984).PubMedCrossRefGoogle Scholar
  15. 15.
    G. W. Burton and M. G. Traber, Vitamin E: antioxidant activity, biokinetics, and bioavailability,Ann. Rev. Nutr. 10, 357–382 (1990).CrossRefGoogle Scholar
  16. 16.
    M. Maiorino, M. Coassin, A. Roveri, and F. Ursini, Microsomal lipid peroxidation: effect of vitamin E and its functional interaction with phospholipid hydroperoxide glutathione peroxidase,Lipids 24, 721–726 (1989).PubMedCrossRefGoogle Scholar
  17. 17.
    G. Haidl and W. B. Schill, Guidelines for drug treatment of male infertility,Drugs 41, 60–68 (1991).PubMedCrossRefGoogle Scholar
  18. 18.
    M. Tolonen, S. Halme, and S. Sarna, Vitamin E and selenium supplementation in geriatric patients: a double blind clinical trial, inSelenium in Biology and Medicine, part B, J. F. Combs, Jr., J. E. Spallholz, O. A. Levander, and J. E. Oldfield, eds., AVI, New York, pp. 701–707 (1987).Google Scholar
  19. 19.
    WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, 3rd. ed., Cambridge University Press, Cambridge (1992).Google Scholar
  20. 20.
    D. Vézina, R. Bélanger, and G. Bleau, Microdetermination of selenium in protein fractions isolated by analytical methods,Biol. Trace Element Res. 24, 153–162 (1990).CrossRefGoogle Scholar
  21. 21.
    H. Verhagen, B. Van Agen, G. J. hageman, R. H. G. Beakers, and J. D. S. Kleinjans, Determination of alpha-tocopherol levels in rat microsomes by high-performance liquid chromatography,J. Liquid Chromatogr. 11, 2977–2982 (1988).CrossRefGoogle Scholar
  22. 22.
    D. E. Paglia and W. N. Valentine, Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase,J. Lab. Clin. Med. 70, 158–169 (1967).PubMedGoogle Scholar
  23. 23.
    M. Borglund and B. Akesson, Effect of selenium supplementation on the distribution of selenium in plasma proteins of healthy subjects,Int. J. Vit. Nutr. Res. 58, 97–102 (1988).Google Scholar
  24. 24.
    G. M. Fairris, B. Lloyd, L. Hinks, P. J. Perkins, and B. E. Clayton, The effect of supplementation with selenium and vitamin E in psoriasis,Ann. Clin. Biochem. 26, 83–88 (1989).PubMedGoogle Scholar
  25. 25.
    H. Sundstrom, H. Korpela, E. Sajanti and A. Kauppilai, Supplementation with selenium, vitamin E and their combination in gynaecological cancer during cytotoxic chemotherapy,Carcinogenesis 10, 273–278 (1989).PubMedCrossRefGoogle Scholar
  26. 26.
    D. G. Smith, P. L. Senger, J. F. McCutchan, and C. A. Landa, Selenium and glutathione peroxidase distribution in bovine semen and selenium-75 retention by the tissues of the reproductive tract in the bull,Biol. Reprod. 20, 377–383 (1979).PubMedCrossRefGoogle Scholar
  27. 27.
    J. L. Bartle, P. L. Senger, and J. K. Hillers, Influence of injected selenium in dairy bulls on blood and semen selenium, glutathione peroxidase and seminal quality,Biol. Reprod. 23, 1007–1013 (1980).PubMedCrossRefGoogle Scholar
  28. 28.
    F. R. Pond, M. J. Tripp, A. S. H. Wu, P. D. Whanger, and J. A. Schmitz, Incorporation of selenium-75 into semen and reproductive tissues of bulls and rams,J. Reprod. Fertil. 69, 411–418 (1983).PubMedGoogle Scholar
  29. 29.
    A. S. H. Wu, J. E. Oldfield, L. R. Shull, and P. R. Cheeke, Specific effect of selenium deficiency on rat sperm,Biol. Reprod. 20, 793–798 (1979).PubMedCrossRefGoogle Scholar
  30. 30.
    E. Wallace, H. I. Calvin, and G. W. Cooper, Progressive defects observed in mouse sperm during the course of three generations of selenium deficiency,Gamete Res. 4, 377–387 (1983).CrossRefGoogle Scholar
  31. 31.
    U. A. Knuth, J. Kuhne, M. Bals-Pratsch, and E. Nieschlag, Intra-individual variation of sperm velocity, linearity, lateral head displacement and beat frequency in healthy volunteers,Andrologia 20, 243–248 (1988).PubMedCrossRefGoogle Scholar
  32. 32.
    K. Purvis, A. Tollefsrud, and H. Rui, Stability of sperm characteristics in men with disturbances in sperm quality,Int. J. Androl. 12, 171–178 (1989).PubMedGoogle Scholar
  33. 33.
    M. Kantola, M. Saaranen, and T. Vanha-Perttula, Selenium and glutathione peroxidase in seminal plasma of men and bulls,J. Reprod. Fertil. 83, 785–794 (1988).PubMedCrossRefGoogle Scholar
  34. 34.
    R. Jones and T. Mann, Damage to ram spermatozoa by peroxidation of endogenous phospholipids,J. Reprod. Fertil. 50, 261–268 (1977).PubMedCrossRefGoogle Scholar
  35. 35.
    J. G. Alvarez and B. T. Storey, Spontaneous lipid peroxidation in rabbit epididymal spermatozoa: its effect on sperm motility,Biol. Reprod. 27, 1102–1108 (1982).PubMedCrossRefGoogle Scholar
  36. 36.
    J. G. Alvarez and B. T. Storey, Assessment of cell damage caused by spontaneous lipid peroxidation in rabbit spermatozoa,Biol. Reprod. 30, 323–331 (1984).PubMedCrossRefGoogle Scholar
  37. 37.
    R. J. Aitken and J. S. Clarkson, Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa,J. Reprod. Fertil. 81, 459–469 (1987).PubMedCrossRefGoogle Scholar
  38. 38.
    R. J. Aitken, J. S. Clarkson and S. Fishel, Generation of reactive oxygen species, lipid peroxidation and human sperm function,Biol. Reprod. 40, 183–197 (1989).CrossRefGoogle Scholar
  39. 39.
    R. J. Aitken, J. S. Clarkson, T. B. Hargreave, D. S. Irvine, and F. C. W. Wu, Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in case of oligozoospermia,J. Androl. 10, 214–220 (1989).PubMedGoogle Scholar
  40. 40.
    J. G. Alvarez and B. T. Storey, Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation,Gamete Res. 23, 77–90 (1989).PubMedCrossRefGoogle Scholar
  41. 41.
    B. Rao, J. C. Soufir, M. Martini, and G. David, Lipid peroxidation in human spermatozoa as related to midpiece abnormalities and motility,Gamete Res. 24, 127–134 (1989).PubMedCrossRefGoogle Scholar
  42. 42.
    J. M. Cummins, A. M. Jequier, and R. Kan, Molecular biology of human male infertility: links with aging, mitochondrial genetics, and oxidative stress?,Mol. Reprod. Dev. 37, 345–362 (1994).PubMedCrossRefGoogle Scholar
  43. 43.
    A. E. Kitabchi, Hormonal status in vitamin E deficiency, inVitamin E: A Comprehensive Treatise, L. J. Machlin, ed., Marcel Dekker Inc, New York, pp. 348–371 (1980).Google Scholar
  44. 44.
    E. C. Wilhelmsen, W. C. Hawkes, M. A. Motsenbocker, and A. L. Tappel, Selenium-containing proteins other than glutathione peroxidase from rat tissue, inSelenium in Biology and Medicine, J. E. Spallholz, J. L. Martin, and H. E. Ganther, eds., AVI Westport, CT, pp. 535–539 (1981).Google Scholar
  45. 45.
    P. D. Whanger, Evidence for non-glutathione peroxidase selenium-containing proteins in mammalian tissues, inSelenium in Biology and Medicine part A G. F. Combs, Jr., J. E. Spallholz, O. A. Levander, and J. E. Oldfield, eds, AVI, New York, pp. 133–146 (1987).Google Scholar
  46. 46.
    A. L. Tappel, Glutathione peroxidase and other selenoproteins, inSelenium in Biology and Medicine Part A, G. F. Combs, Jr., J. E. Spallholz, O. A. Levander, and J. E. Oldfield, eds., AVI, New York, pp. 122–132 (1987).Google Scholar
  47. 47.
    B. Akesson and M. Borglund, Effect of selenium supplementation on the concentration and distribution of selenium in leucocytes erythrocytes and plasma of human subjects, inProceedings of the Fourth International Symposium on Uses of Selenium and Tellurium, S. C. Carapella, Jr., ed., SIDA, Darien, CT, pp. 405–419 (1989).Google Scholar
  48. 48.
    P. D. Whanger, J. A. Butler, C. D. Thomson, X. Zhao, and Y. Xia, Selenium metabolism studies in humans living in the United States, New Zealand and China, inProceedings of the Fourth International Symposium on Uses of Selenium and Tellurium, S. C. Carapella, Jr. ed., STDA, Darien, CT, pp. 361–377 (1989).Google Scholar
  49. 49.
    S. Van Erum, P. Capel, F. Vertongen, and J. Neve, Effects of selenium supplementation with Se enriched yeast tablets on hepatic, muscular, real and hematological parameters in humans, inSelenium in Medicine and Biology, J. Neve and A. Favier, eds., Walter de Gruyter, Berlin, pp. 321–324 (1988).Google Scholar
  50. 50.
    R. S. Hotchkiss, Factors in stability and variability of semen observations on 640 successive samples from 23 men,J. Urol. 45, 875 (1941).Google Scholar
  51. 51.
    J. C. Czyba, M. C. Pinatel, and C. Souchier, Variations saisonnières dans la composition cellulaire du sperme humainSem. Hôp. Paris 55, 596–598 (1979).PubMedGoogle Scholar
  52. 52.
    D. Mortimer, A. A. Templeton, E. A. Lenton, and R. A. Coleman, Annual patterns of human sperm production and semen quality,Arch. Androl. 10, 1–7 (1983).PubMedCrossRefGoogle Scholar
  53. 53.
    R. J. Levine, M. H. Brown, B. L. Bordson, J. M. Stanley, R. M. Mathew, and T. B. Starr, Deterioration of semen quality during summer in New Orleans,Fertil. Steril. 49, 900–907 (1988).PubMedGoogle Scholar
  54. 54.
    A. Reinberg, K. D. Smith, M. H. Smolensky, E. Steinberger, and M. Hallek, Annual variation in semen characteristics and plasma hormone levels in men undergoing vasectomy,Fertil. Steril. 49, 309–315 (1988).PubMedGoogle Scholar
  55. 55.
    L. Politoff, M. Birkhauser, A. Almendral, and A. Zorn, New data confirming a circannual rthythm in spermatogenesis,Fertil. Steril. 52, 486–489 (1989).PubMedGoogle Scholar
  56. 56.
    P. Saint Pol, E. Hermand, R. Beuscart, W. Jablonski, and M. Leroy-Martin, Circannual rhythms of sperm parameters of fertile men,Fertil. Steril. 51, 1030–1033 (1989).PubMedGoogle Scholar
  57. 57.
    H. W. G. Baker, H. G. Burger, D. M. DeKretser, D. W. Lording, P. McGowan, and G. C. Rennie, Factors affecting the variability of semen analysis results in infertile men,Int. J. Androl. 4, 609–622 (1981).PubMedGoogle Scholar
  58. 58.
    R. Z. Sokol, G. Petersen, B. S. Steiner, R. S. Swerdloff, and M. Bustillo, A controlled comparison of the efficacy of clomiphene citrate in male infertility,Fertil. Steril. 49, 865–870 (1988).PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Denis Vézina
    • 1
    • 5
  • François Mauffette
    • 2
    • 4
  • Kenneth D. Roberts
    • 3
    • 5
  • Gilles Bleau
    • 1
    • 5
  1. 1.Department of Obstetrics and GynecologyUniversity of MontrealMontrealCanada
  2. 2.Department of MedicineUniversity of MontrealMontrealCanada
  3. 3.Department of BiochemistryUniversity of MontrealMontrealCanada
  4. 4.St-Luc HospitalMontrealCanada
  5. 5.Maisonneuve-Rosemont Research CenterMontrealCanada

Personalised recommendations