Advertisement

Cell Biochemistry and Biophysics

, Volume 7, Issue 2, pp 91–105 | Cite as

Microscope laser light scattering spectroscopy of single biological cells

  • I. Nishio
  • J. Peetermans
  • T. Tanaka
Original Articles

Abstract

A microscope laser light scattering setup was developed, allowing us to do intensity autocorrelation spectroscopy on the light scattered from a volume as small as (2 μm)3. This non-invasive technique makes cytoplasmic studies possible inside single live biological cells. The effect of osmotic swelling and shrinking on the diffusion coefficient of hemoglobin inside intact red blood cells is shown as an illustrative example of the applicability and sensitivity of this new experimental method.

Index Entries

Microscope laser light scattering spectroscopy, of cells laser light scattering spectroscopy, of cells light scattering spectroscopy, of cells by laser microscopy spectroscopy of cells, microscope laser light scattering cells, laser light scattering spectroscopy of 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Lansing Taylor, D., and Wang, Y.-L. (1980)Nature 284, 405.CrossRefGoogle Scholar
  2. 2.
    Maeda, T., and Fujime, S. (1972)Rev. Sci. Instr. 43, 566.CrossRefGoogle Scholar
  3. 3.
    Nishio, I., Tanaka, T., Sun, S.-T., Imanishi, Y., and Ohnishi, S. T. (1983)Science 220, 173.CrossRefGoogle Scholar
  4. 4.
    Sun, S.-T., Tanaka, T., Nishio, I., Peetermans, J., Maizel, J. V., Jr., and Piatigorsky, J., (1984)Proc. Natl. Acad. Sci. USA 81, 785.PubMedCrossRefGoogle Scholar
  5. 5.
    Benedek, G. B. (1969),Polarization, Matter and Radiation, Presses Universitaires de France, Paris, pp. 49–84.Google Scholar

Copyright information

© The Humana Press Inc 1985

Authors and Affiliations

  • I. Nishio
    • 1
  • J. Peetermans
    • 2
  • T. Tanaka
    • 2
  1. 1.Center for Polymer Studies, Department of PhysicsBoston UniversityBoston
  2. 2.Department of Physics and Center for Materials Science and EngineeringMassachusetts Institute of TechnologyCambridge

Personalised recommendations