Cell Biochemistry and Biophysics

, Volume 7, Issue 2, pp 91–105 | Cite as

Microscope laser light scattering spectroscopy of single biological cells

  • I. Nishio
  • J. Peetermans
  • T. Tanaka
Original Articles


A microscope laser light scattering setup was developed, allowing us to do intensity autocorrelation spectroscopy on the light scattered from a volume as small as (2 μm)3. This non-invasive technique makes cytoplasmic studies possible inside single live biological cells. The effect of osmotic swelling and shrinking on the diffusion coefficient of hemoglobin inside intact red blood cells is shown as an illustrative example of the applicability and sensitivity of this new experimental method.

Index Entries

Microscope laser light scattering spectroscopy, of cells laser light scattering spectroscopy, of cells light scattering spectroscopy, of cells by laser microscopy spectroscopy of cells, microscope laser light scattering cells, laser light scattering spectroscopy of 


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Copyright information

© The Humana Press Inc 1985

Authors and Affiliations

  • I. Nishio
    • 1
  • J. Peetermans
    • 2
  • T. Tanaka
    • 2
  1. 1.Center for Polymer Studies, Department of PhysicsBoston UniversityBoston
  2. 2.Department of Physics and Center for Materials Science and EngineeringMassachusetts Institute of TechnologyCambridge

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