Biological Trace Element Research

, Volume 62, Issue 3, pp 167–182 | Cite as

Preparation of hair for measurement of elements by inductively coupled plasma-mass spectrometry (ICP-MS)

  • Richard F. Puchyr
  • Dean A. Bass
  • Rob Gajewski
  • Mishelle Calvin
  • William Marquardt
  • Karen Urek
  • Mary Ellen Druyan
  • David Quig
Article

Abstract

The preparation of hair for the determination of elements is a critical component of the analysis procedure. Open-beaker, closedvessel microwave, and flowthrough microwave digestion are methods that have been used for sample preparation and are discussed. A new digestion method for use with inductively coupled plasma-mass spectrometry (ICP-MS) has been developed. The method uses 0.2 g of hair and 3 mL of concentrated nitric acid in an atmospheric pressurelow-temperature microwave digestion (APLTMD) system. This preparation method is useful in handling a large numbers of samples per day and may be adapted to hair sample weights ranging from 0.08 to 0.3 g. After digestion, samples are analyzed by ICP-MS to determine the concentration of Li, Be, B, Na, Mg, Al, P, S, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ge, As, Se, Rb, Sr, Zr, Mo, Pd, Ag, Cd, Sn, Sb, I, Cs, Ba, Pt, Au, Hg, Tl, Pb, Bi, Th, and U. Benefits of the APLTMD include reduced contamination and sample handling, and increased precision, reliability, and sample throughput.

Index Entries

Hair elements Li, Be, B, Na, Mg, AI, P, S, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ge, As, Se, Rb, Sr, Zr, Mo, Pd, Ag, Cd, Sn, Sb, I, Cs, Ba, Pt, Au, Hg, Tl, Pb, Bi, Th, and U microwave digestion ICP-MS 

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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Richard F. Puchyr
    • 1
  • Dean A. Bass
    • 1
  • Rob Gajewski
    • 1
  • Mishelle Calvin
    • 1
  • William Marquardt
    • 1
  • Karen Urek
    • 1
  • Mary Ellen Druyan
    • 1
  • David Quig
    • 1
  1. 1.Doctor’s Data Inc.West Chicago

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