Applied Biochemistry and Biotechnology

, Volume 49, Issue 3, pp 203–215 | Cite as

Immobilization, characterization, and laboratory-scale application of bovine liver arginase

  • Erzs’ebet Dala
  • B. Szaj’ani
Article

Abstract

Arginase isolated from beef liver was covalently attached to a polyacrylamide bead support bearing carboxylic groups activated by a water-soluble carbodiimide. The most favorable carbodiimide wasN-cyclohexyl-Nt’-(methyl-2-p-nitrophenyl-2-oxoethyl) aminopropyl carbodiimide methyl bromide, but for practical purposes,N-cyclohexyl-Nt’-morpholinoethyl carbodiimide methyl tosylate was used. The optimal conditions for the coupling procedure were determined. The catalytic activity of the immobilized arginase was 290–340 U/g solid or 2.9–3.4 U/mL wet gel. The pH optimum for the catalytic activity was pH 9.5, the apparent temperature maximum was at 60°C and Kmapp was calculated to be 0.37M L-arginine. Immobilization markedly improved the conformational stability of arginase. At 60°C, the pH for maximal stability was found to be 8.0. The immobilized arginase was used for the production of L-ornithine and D-arginine.

Index Entries

Arginase, immobilized support, polyacrylamide bead carbodiimide, coupling agent immobilized arginase, properties L-ornithine, production D-arginine, production. 

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Copyright information

© Humana Press Inc 1994

Authors and Affiliations

  • Erzs’ebet Dala
    • 1
  • B. Szaj’ani
    • 1
  1. 1.Reanal Factory of Laboratory ChemicalsBudapest 70Hungary

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