Hepatic collagen synthesis in patients with alcoholic and nonalcoholic liver disease
- 36 Downloads
To examine the synthesis of hepatic collagen in patients with alcoholic and nonalcoholic liver disease, liver biopsy specimens were incubated in vitro with14C-proline, and the radioactivity of the newly synthesized protein-bound14C-hydroxyproline was measured. Mean hepatic collagen synthesis was 0.82±0.19 pmole of14C-hydroxyproline/g liver/2 h in control subjects without histological liver fibrosis. Hepatic collagen synthesis was increased in patients with alcoholic and nonalcoholic liver diseases, especially in those with alcoholic fibrosis, alcoholic cirrhosis and chronic active hepatitis. The raised collagen synthesis in alcoholic liver disease rapidly decreased after withdrawal of alcohol. When alcoholic liver disease were compared with nonalcoholic liver disease, there was no significant difference in hepatic collagen synthesis.
Key wordsalcoholic liver disease hepatic collagen synthesis nonalcoholic liver disease
Unable to display preview. Download preview PDF.
- 7.Rojkind M, Gonzalez E: An improved method for determining specific radioactivities of proline-l4C and hydroxyproline-14C in collagen and in noncollagenous proteins. Analy Biochem 1974;54:l-7.Google Scholar
- 8.Murawaki Y, Kato S, Koda M et al: Hepatic collagen degrada-tion system in early stage of ethanol-induced liver injury. In: C Hirayama, KI Kivirikko, eds. Pathobiology of hepatic fibrosis. Excerpta Medica, Amsterdam. 1985;121–129.Google Scholar
- 12.Kershemobich D, Fierro FJ, Rojkind M: The relationship be-tween the free pool of proline and collagen content in human liver dirrhosis. J Clin Invest 1970;49:2246–2249.Google Scholar
- 16.Tseng SCG, Smuckler EA, Stern R: Types of collagen synthe-sized by normal rat liver hepatocytes in primary culture. Hep-atology 1983;3:955–963.Google Scholar
- 21.Kimura K: Clinical and experimental studies on liver prolyl hydroxylase at various atages of liver disease. J Nara Med Ass 1980;31:76–89. (in Japanese)Google Scholar
- 24.Stern R, Tseng SCG: The fibrosis of chronic alcoholic liver dis-ease. In: PD Berk, TC Chalmers, ets. Frontiers in Liver Dis-ease. Thieme-Stratton, New York 1981:144–155.Google Scholar