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Gastroenterologia Japonica

, Volume 26, Issue 1, pp 29–36 | Cite as

Kinetic changes of liver regeneration and hepatocellular carcinoma cells after partial hepatectomy in rats

  • Tsutomu Namieno
  • Noritoshi Takeichi
  • Yoshinobu Hata
  • Junichi Uchino
  • Hiroshi Kobayashi
Liver, Pancreas and Biliary Tract

Summary

We investigated, using rats, the effect of partial hepatectomy (PH) on hepatocellular carcinoma (HCC, KDH-8 and AH-66) cells, and the effect of HCC cells on the regeneration of remaining hepatocytes after PH. Our results showed that PH significantly enhanced the growth of HCC cells in rats. Tumor volume increased more significantly in the partially hepatectomized group (H-group) than in the control group, and the tumor wet weights on the 14th postoperative day were significantly higher in the H-group than in the control group. Such an enhanced growth effect of PH on the injected (s.c) HCC cells was related to an abrupt increase of tumor volume within 24 hours after operation, which was supported by the mitotic indices (MI) of the KDH-8 cells. These phenomena of the enhanced growth of the HCC cells following PH were not observed at all in rats injected with estrogen receptor (ER)-negative mammary carcinoma (SST-2) or nonepithelial fibrosarcoma (KMT-75) cells. The MIs of the remaining hepatocytes after PH increased abruptly at the 30th postoperative hour and reached a maximum at the 36th postoperative hour, and the MIs were significantly higher in the H-group with the KDH-8 cells than in the H-group without them from the 42th to the 60th postoperative hour. In the control group, the MIs of hepatocytes were not regardless of the presence of KDH-8 cells. From these results, we speculate that some growth factor(s) induced by PH may act on injected (s.c.) HCC cells, and that the other growth factor(s) secreted by HCC cells may act on the regenerating hepatocytes after PH.

Key words

partial hepatectomy liver regeneration liver cancer growth growth factor 

Abbreviations used in this paper

HCC

hepatocellular carcinoma

PH

partial (70%) hepatectomy

H-group

partially hepatectomized group

S-group

sham-operated group

N-group

non-operated group

MI

mitotic index

ER

estrogen receptor

WKA/H

Wistar King Aptekman/Hok

SHR

Spontaneously Hypertensive Rat

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Copyright information

© The Japanese Society of Gastroenterology 1991

Authors and Affiliations

  • Tsutomu Namieno
    • 1
    • 2
  • Noritoshi Takeichi
    • 1
  • Yoshinobu Hata
    • 2
  • Junichi Uchino
    • 2
  • Hiroshi Kobayashi
    • 1
  1. 1.Laboratory of Pathology, Cancer InstituteHokkaido University School of MedicineHokkaidoJapan
  2. 2.First Department of SurgeryHokkaido University School of MedicineSapporoJapan

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