Applied Biochemistry and Biotechnology

, Volume 17, Issue 1–3, pp 357–369 | Cite as

Production of ethanol from sugar cane bagasse hemicellulose hydrolyzate byPichia stipitis

Scientific note
  • Carina Van Zyl
  • Bernard A. Prior
  • James C. Du Preez
Session 2 Scientific Notes

Abstract

The ability ofPichia stipitis to fermentd-xylose andd-glucose in the acid-hydrolyzed hemicellulose component of sugar cane bagasse depends on the alkali used to neutralize the hydrolyzate to pH 6.5. With NH4OH and NaOH no fermentation occurred, whereas neutralization with Ca(OH)2 gave the best results (Qpmax=0.25 g/L-h; Yp/s =0.38 g/g sugar). However, the volumetric productivity was still considerably less than observed in a semisynthetic medium with a sugar composition similar to the hydrolyzate. L-arabinose was not fermented but assimilated. Sequential neutralization methods failed to improve the fermentation. Acetic acid and lignin derivatives present in the hydrolyzate were major components that inhibited the fermentation.

Index Entries

Hemicellulose hydrolyzate d-xylose fermentation ethanol production inhibitors Pichia stipitis neutralization techniques 

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References

  1. 1.
    Dekker, R. F. H., and Lindner, W. A. (1979),S. Afr. J. Sci. 75, 65.Google Scholar
  2. 2.
    Schneider, H., Wang, P. Y., Chan, Y. K., and Maleszka, R. (1981).Biotechnol. Lett. 3, 89.CrossRefGoogle Scholar
  3. 3.
    Lee, Y. Y., and McCaskey, T. A. (1983),Tappi J. 66, 102.Google Scholar
  4. 4.
    Watson, N. E., Prior, B. A., Lategan, P. M., and Lussi, M. (1984),Enzyme Microb. Technol. 6, 451.CrossRefGoogle Scholar
  5. 5.
    Hajny, G. I. (1981),Biological utilization of wood for production of chemicals and foodstuffs. US Dept. Agric. For. Serv. For. Prod. Lab., Madison, WI, Research Paper FPL 385.Google Scholar
  6. 6.
    Magee, R. J., and Kosaric, N. (1985),Adv. Biochem. Eng./Biotechnol. 32, 61.Google Scholar
  7. 7.
    Jeffries, T. W. (1985),Trends Biotechnol 3, 208.bCrossRefGoogle Scholar
  8. 8.
    Du Preez, J. C., and Prior, B. A. (1985),Biotechnol. Lett. 7, 241.CrossRefGoogle Scholar
  9. 9.
    Dellweg, H., Rizzi, M., Methner, H., and Debus, D. (1984),Biotechnol. Lett. 6, 395.CrossRefGoogle Scholar
  10. 10.
    Du Preez, J. C., Bosch, M., and Prior, B. A. (1986),Appl. Microbiol. Biotechnol. 23, 228.CrossRefGoogle Scholar
  11. 11.
    Wickerham, L. J. (1951),Taxonomy of yeasts. US Dept. Agric., Washington, DC, Tech. Bull. No. 1029.Google Scholar
  12. 12.
    Strickland, R. C., and Beck, M. J. (1984), Effective pretreatments and neutralization methods for ethanol production from acid-catalyzed hardwood hydrolyzates usingPachysolen tannophilus.Proc. 6th Int. Symp. Alcohol Fuels Technol., Ottawa, Canada, vol. 2, pp. 220–226.Google Scholar
  13. 13.
    Du Preez, J. C., and van der Walt, J. P. (1983),Biotechnol. Lett. 5, 357.CrossRefGoogle Scholar
  14. 14.
    Gong, C-S., and Tsao, G. T. (1982), Production of ethanol from biomass hemicellulose hydrolyzate by yeasts.Proc. Natl. Meet. Am. Inst. Chem. Eng., Orlando, Florida, Paper No. 43, pp. 1–17.Google Scholar
  15. 15.
    Du Preez, J. C., Bosch, M., and Prior, B. A. (1986),Enzyme Microb. Technol. 8, 360.CrossRefGoogle Scholar
  16. 16.
    Weast, R. C., Selby, S. M., and Hodgeman, C. D., eds. (1965),Handbook of Chemistry and Physics, 46th ed., Chemical Rubber Co., Cleveland, OH, p. D-79.Google Scholar
  17. 17.
    Vogel, A. I. (1961),A Textbook of Quantitative Inorganic Analysis, 3rd ed., Longmans, Green & Co. Ltd., London, pp. 1144–1145.Google Scholar
  18. 18.
    Šestáková, M. (1979),Folia Microbiol. 24, 318.CrossRefGoogle Scholar
  19. 19.
    Tran, A. V., and Chambers, R. P. (1985),Biotechnol. Lett. 7, 841.CrossRefGoogle Scholar
  20. 20.
    Tran, A. V., and Chambers, R. P. (1986),Enzyme Microb. Technol. 8, 439.CrossRefGoogle Scholar
  21. 21.
    Noller, C. R. (1958),Textbook of Organic Chemistry, Saunders, Philadelphia, PA, pp. 457–460.Google Scholar

Copyright information

© The Humana Press Inc 1988

Authors and Affiliations

  • Carina Van Zyl
    • 1
  • Bernard A. Prior
    • 1
  • James C. Du Preez
    • 1
  1. 1.Department of MicrobiologyUniversity of the Orange Free StateBloemfonteinSouth Africa

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