Applied Biochemistry and Biotechnology

, Volume 17, Issue 1–3, pp 163–173

Mutants ofEscherichia coli defective in acid fermentation

  • David P. Clark
  • Philip R. Cunningham
  • Stephen G. Reams
  • Fairoz Mat-Jan
  • Raafat Mohammedkhani
  • Charling R. Williams
Session 2 Original Papers

Abstract

Wild typeE. coli ferments glucose to a mixture of ethanol and acetic, lactic, formic, and succinic acids. Mutants defective in acid production have now been isolated, including those defective in lactate dehydrogenase (LDH) or with excess alcohol dehydrogenase. These mutations had no phenotype without apfl mutation. Novel mutants affecting acetate metabolism were isolated by insertion of the fusion vector Mudl. TheseaceG mutants cannot grow anaerobically on glucose or aerobically on acetate yet lack the pleiotropic growth defects of previously knownpta/ack mutants. In some genetic backgrounds acetate negative mutations suppress the growth defects ofadh mutations. These results are discussed in terms of redox balance.

Index Entries

Anaerobic growth alcohol dehydrogenase factate fermentation acetic acid, redox balance 

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References

  1. 1.
    Clark, D. P. and Cronan, J. E. (1980),Escherichia coli mutants with alter control of alcohol dehydrogenase and nitrate reductase.J. Bacteriol. 141, 177–183.Google Scholar
  2. 2.
    Lorowitz, W. and Clark, D. P. (1982),Escherichia coli mutants with a temperature-sensitive alcohol dehydrogenase.J. Bacteriol. 152, 935–938.Google Scholar
  3. 3.
    Cunningham, P. R. and Clark, D. P. (1986), The use of suicide substrates to isolate mutants ofEscherichia coli lacking enzymes of alcohol fermentation.Mol. Gen. Genet. 205, 487–493.CrossRefGoogle Scholar
  4. 4.
    Guest, J. R. (1979), Anaerobic growth ofEscherichia coli K-12 with fumarate as terminal electron acceptor. Genetic studies with menaquinone and fluoroacetate resistant mutants.J. Gen. Microbiol. 115, 259–271.Google Scholar
  5. 5.
    Vogel, H. J. and Bonner, D. M. (1956), Acetylornithinase inEscherichia coli.J. Biol. Chem. 218, 97–103.Google Scholar
  6. 6.
    Winkelman, J. W. and Clark, D. P. (1984), Proton suicide: General method for direct selection of sugar transport and fermentation-defective mutants.J. Bacteriol. 160, 687–690.Google Scholar
  7. 7.
    Miller, J. H. (1972),Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.Google Scholar
  8. 8.
    Tarmy, E. M. and Kaplan, N. O. (1968), Chemical characterization of D-lactate dehydrogenase fromEscherichia coli.J. Biol. Chem. 243, 2579–2586.Google Scholar
  9. 9.
    Winkelman, J. W. and Clark, D. P. (1986), Anaerobically induced genes ofEscherichia coli.J. Bacteriol. 167, 362–367.Google Scholar
  10. 10.
    LeVine, N. M., Ardeshir, F. and Ames, G. F-L. (1980), Isolation and characterization of acetate kinase and phosphotransacetylase mutants ofEscherichia coli andSalmonella typhimurium.J. Bacteriol. 143, 1081–1085.Google Scholar
  11. 11.
    Bachmann, B. (1983), Linkage map ofEscherichia coli K12, edition 7.Microbiol. Rev. 47, 180–230.Google Scholar
  12. 12.
    Clark, D. P. (1981), Regulation of fatty acid degradation inEscherichia coli: Analysis by operon fusion.J. Bacteriol. 148, 521–526.Google Scholar
  13. 13.
    Clark, D. P. (1984), The number of anaerobically regulated genes inEscherichia coli.FEMS Microbiol. Lett. 24, 251–254.CrossRefGoogle Scholar
  14. 14.
    Varenne, S., Casse, F., Chippaux, M. and Pascal, M. C. (1975), A mutant ofEscherichia coli deficient in pyruvate formate lyase.Molec. Gen. Genet. 141, 181–184.CrossRefGoogle Scholar
  15. 15.
    Ingram, L. O., Conway, T. Clark, D. P., Sewell, G. S., and Preston, J. F. (1987), Genetic engineering of ethanol production inEscherichia coli J. Appl. Environ. Microbiol. In press.Google Scholar

Copyright information

© The Humana Press Inc 1988

Authors and Affiliations

  • David P. Clark
    • 1
  • Philip R. Cunningham
    • 1
  • Stephen G. Reams
    • 1
  • Fairoz Mat-Jan
    • 1
  • Raafat Mohammedkhani
    • 1
  • Charling R. Williams
    • 1
  1. 1.Department of MicrobiologySouthern Illinois UniversityCarbondale

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