On the successful treatment of pancreatitis with chlorophyll-a and inhibitory effects of its derivatives on trypsin and other protease activitiesin vitro
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Patients with pancreatitis, in particular with chronic relapsing pancreatitis have recently been experienced by the authors, in which chlorophyll-a intravenously administered showed a marked therapeutic effect. Both fever and abdominal colicky pain were relieved shortly, and remarkably high levels of amylase activity in their urine returned to normal, after administration.
In a series ofin vitro experiments performed, it has been proved that chlorophyllin (a phytol group is removed from chlorophyll-a) exhibited an inhibitory effect on trypsin activity at the minimum concentration of 5×l0-6M, and 50 per cent inhibition was seen at 9×10-5M.
On some other proteases, such as pepsin, α-chymotrypsin and kallikrein except for elastase, a more or less inhibition was also shown.
Chlorophyll-a exerted, however, no effect at all,in vitro.
Derivatives of chlorophyllin in which Fe, Co, Cu or Zn are chelated in place of its Mg, also showed a similar effect, while phytochlorin losing its metal from chlorophyllin, showed only a slight inhibition.
From these it is most likely that chlorophyll-a is a prospective agent for treating pancreatitis, particularly chronic relapsing pancreatitis, in which even a long-term administration is to be needed, because it has no antigenicity in nature.
Key wordsPancreatitis Chlorophyll-a Protease activities
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