Advertisement

Gastroenterologia Japonica

, Volume 6, Issue 2, pp 49–54 | Cite as

On the successful treatment of pancreatitis with chlorophyll-a and inhibitory effects of its derivatives on trypsin and other protease activitiesin vitro

  • Toshitsugu Oda
  • Osamu Yokono
  • Akira Yoshida
  • Kazuhiko Miyake
  • Shiro Iino
Original Article
  • 20 Downloads

Summary

Patients with pancreatitis, in particular with chronic relapsing pancreatitis have recently been experienced by the authors, in which chlorophyll-a intravenously administered showed a marked therapeutic effect. Both fever and abdominal colicky pain were relieved shortly, and remarkably high levels of amylase activity in their urine returned to normal, after administration.

In a series ofin vitro experiments performed, it has been proved that chlorophyllin (a phytol group is removed from chlorophyll-a) exhibited an inhibitory effect on trypsin activity at the minimum concentration of 5×l0-6M, and 50 per cent inhibition was seen at 9×10-5M.

On some other proteases, such as pepsin, α-chymotrypsin and kallikrein except for elastase, a more or less inhibition was also shown.

Chlorophyll-a exerted, however, no effect at all,in vitro.

Derivatives of chlorophyllin in which Fe, Co, Cu or Zn are chelated in place of its Mg, also showed a similar effect, while phytochlorin losing its metal from chlorophyllin, showed only a slight inhibition.

From these it is most likely that chlorophyll-a is a prospective agent for treating pancreatitis, particularly chronic relapsing pancreatitis, in which even a long-term administration is to be needed, because it has no antigenicity in nature.

Key words

Pancreatitis Chlorophyll-a Protease activities 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    Anson, M.L.: J. Gen. Physiol. 22: 79, 1938, In Methods in Enzymology, Vol. 11, p. 34, S.P. Colowick and N.O. Kaplan, Ed. Acad. Press, N.Y., 1955.CrossRefGoogle Scholar
  2. 2).
    Hestrin, S.: J. Biol. Chem. 180: 249, 1949.Google Scholar
  3. 3).
    Hall, D.A.: Biochem. J. 101: 29, 1966.PubMedGoogle Scholar
  4. 4).
    Shindo, T., Haga, K., Watanabe, T., Fujii, G., and Nishioka, K.: Jap. J. Exptl. Med. 36: 489, 1966.Google Scholar

Copyright information

© The Japanese Society of Gastroenterology 1971

Authors and Affiliations

  • Toshitsugu Oda
    • 1
    • 2
  • Osamu Yokono
    • 1
  • Akira Yoshida
    • 1
  • Kazuhiko Miyake
    • 1
  • Shiro Iino
    • 1
  1. 1.The First Department of Medicine, Faculty of MedicineUniversity of TokyoTokyoJapan
  2. 2.Faculty of MedicineUniversity of TokyoTokyoJapan

Personalised recommendations