Advertisement

Gastroenterologia Japonica

, Volume 27, Issue 1, pp 23–28 | Cite as

Expression of MHC class II antigens (HLA-DR, -DP, and -DQ) on human gastric epithelium

  • Nobuaki Ishii
  • Mitsuro Chiba
  • Masahiro Iizuka
  • Hiroyuki Watanabe
  • Tomonori Ishioka
  • Osamu Masamune
Alimentary Tract

Summary

Class II antigen expression on gastric epithelium was investigated using an immunoperoxidase method in relation to the degree of inflammatory cell infiltration in the lamina propria. Sixty-six biopsy specimens from 43 patients with chronic gastritis were examined. The frequency of HLA-DR expression in specimens with cell infiltration was 94%, while that in specimens without cell infiltration was 24%. There was significant difference in the frequency of HLA-DR expression between the two groups (P<0.01). HLA DR fwas most intensely expressed in the glandular neck portion. The frequency and extent of class II antigen expression on gastric epithelium with cell infiltration were in the following order: DR>DP>DQ. The extent of DR and DP, but not DQ expression generally paralleled the degree of cell infiltration. Intestinal metaplasia was found in 13 specimens. In the area of intestinal metaplasia, epithelial class II staining was absent except for one specimen. These results suggest that the respectivegenes of three class II antigens are regulated by different mechanisms and that an immunological mechanism plays a role in the pathogenesis of gastritis.

Key words

class II antigens gastric epithelium HLA-DR HLA-DP HLA-DQ 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Thorsby E: Structure and function of HLA molecules. Transplantation Proc 1987;19:29–35.Google Scholar
  2. 2.
    Steinman RM: Dendritic cells. Transplantation 1981;31:151–155.PubMedCrossRefGoogle Scholar
  3. 3.
    Daar AS, Fuggle SV, Fabre JW, et al: The detailed distribution of MHC class II antigens in normal human organs. Transplantation 1984;38:293–298.PubMedGoogle Scholar
  4. 4.
    Unanue ER: Antigen-presenting function of the macrophage. Ann Rev Immunol 1984;2:395–428.CrossRefGoogle Scholar
  5. 5.
    Wiman K, Curman B, Forsum U, et al: Occurrence of la antigens on tissues on non-lymphoid origin. Nature 1978;276:711–713.PubMedCrossRefGoogle Scholar
  6. 6.
    Natali PG, De Màrtino C, Quaranta V, et al: Expression of la-like antigens in normal human nonlymphoid tissues. Transplantation 1981;31:75–78.PubMedCrossRefGoogle Scholar
  7. 7.
    Scott H, Sollid LM, Fausa O, et al: Expression of major histocompatibility complex class II subregion products by jejunal epithelium in patients with coeliac disease. Scand J Immunol 1987;26: 563–571.PubMedCrossRefGoogle Scholar
  8. 8.
    Marley NJE, Macartney JC, Ciclitira PJ: HLA-DR, DP, and DQ expression in the small intestine of patients with coeliac disease. Clin exp Immunol 1987;70:386–393.PubMedGoogle Scholar
  9. 9.
    Chiba M. Iizuka M, Masamune, O: Ubiquitous expression of HLA-DR antigens on human small intestinal epithelium. Gastroenterol Jpn 1988;23:109–116.PubMedGoogle Scholar
  10. 10.
    Iizuka M, Chiba M, Ohta H, et al: Expression of HLA-DR antigens on colonic epithelium in ulcerative colitis. Gastroenterol Jpn 1987;22:571–577.PubMedGoogle Scholar
  11. 11.
    Selby WS, Janossy G, Mason DY, et al: Expression of HLA-DR antigens by colonic epithelium in inflammatory bowel disease. Clin exp Immunol 1983;53:614–618.PubMedGoogle Scholar
  12. 12.
    Hirata I, Austin LL, Blackweel WH, et al: Immunoelectron microscopic localization of HLA-DR antigen in control small intestine and colon and inflammatory bowel disease. Dig Dis Sci 1986;31:1317–1330.PubMedCrossRefGoogle Scholar
  13. 13.
    Fais S, Pallone F, Squarcia O, et al: HLA-DR antigens on colonie epithelial cells in inflammatory bowel disease: I. Relation to the state of activation of lamina propria lymphocytes and to the epithelial expression of other surface markers. Clin exp Immunol 1987; 68:605–612.PubMedGoogle Scholar
  14. 14.
    McDonald GB, Jewell DP: Class II antigen (HLA-DR) expression by intestinal epithelial cells in inflammatory diseases of colon. J Clin Pathol 1987;40:312–317.PubMedCrossRefGoogle Scholar
  15. 15.
    Ouyang Q, El-Youssef M, Yen-Lieberman B, et al: Expression of HLA-DR antigens in inflammatory bowel disease mucosa: Role of intestinal lamina propria mononuclear cell-derived interferon y. Dig Dis Sci 1988;33:1528–1536.CrossRefGoogle Scholar
  16. 16.
    Chiba M, Iizuka M, Horie Y, et al: HLA-DR antigens expression in macroscopically uninvolved areas of intestinal epithelia in Crohn’s disease. Gastroenterol Jpn 1989;24:365–372.PubMedGoogle Scholar
  17. 17.
    Horie Y, Chiba M, Iizuka M, et al: Class II (HLA-DR, -DP, and -DQ) antigens on intestinal epithelia in ulcerative colitis, Crohn’s disease, colorectal cancer and normal small intestine. Gastroenterol Jpn 1990;25:575–584.PubMedGoogle Scholar
  18. 18.
    Spencer J, Finn T, Isaacson PG: Expression of HLA-DR antigens on epithelium associated with lymphoid tissue in the human gastrointestinal tract. Gut 1986;27:153–157.PubMedCrossRefGoogle Scholar
  19. 19.
    Sakai K, Takiguchi M, Mori S, et al: Expression and function of class II antigens on gastric carcinoma cells and gastric epithelia: Differential expression of DR, DQ, and DP antigens. J Natl Cancer Inst 1987;79:923–932.PubMedGoogle Scholar
  20. 20.
    Papadimitriou CS, Ioachim-Velogianni EE, Tsianos EB, et al: Epithelial HLA-DR expression and lymphocyte subsets in gastric mucosa in type B chronic gastritis. Virchows Arch [A] 1988;413: 197–204.Google Scholar
  21. 21.
    Vaines K, Huitfeldt HS, Brandtzaeg P: Relation between T cell number and epithelial HLA class II expression quantified by image analysis in normal and inflamed human gastric mucosa. Gut 1990;31:647–652.CrossRefGoogle Scholar
  22. 22.
    Chiba M, Ohta H, Nagasaki A, et al: Lymphoid cell subsets in normal human small intestine. Gastroenterol Jpn 1986;21:336–343.PubMedGoogle Scholar
  23. 23.
    Matsumoto K: Studies of monoclonal antibodies specific to human leukocyte differentiation antigens. J Kurume Med Assoc 1984;47:1382–1397. (Japanese with English abstract)Google Scholar
  24. 24.
    Rao SS, Krasner N, Thomson TJ: Chronic gastritis—a simple classification. J Pathol 1975;117:93–96.PubMedCrossRefGoogle Scholar
  25. 25.
    Ghosh AK, Moore M, Street AJ, et al: Expression of HLA-D subregion products on human colorectal carcinoma. Int J Cancer 1986;38:459–464.PubMedCrossRefGoogle Scholar
  26. 26.
    Degener T, Monburg F, Möller P: Differential expression of HLA-DR, HLA-DP, HLA-DQ and associated invariant chain (Ii) in normal colorectal mucosa, adenoma and carcinoma. Virchows Arch [A] 1988;412:315–322.CrossRefGoogle Scholar
  27. 27.
    Guy K, Krajewski AS, Dewar AK: Expression of MHC class II antigens in human B-cell leukemia and non-Hodgkin’s lymphoma. Br J Cancer 1986;53:161–173.PubMedGoogle Scholar
  28. 28.
    Hirayama K, Matsushita S, Kikuchi I, et al: HLA-DQ is epistatic to HLA-DR in controlling the immune response to schistosomal antigen in humans. Nature 1987;327:426–430.PubMedCrossRefGoogle Scholar
  29. 29.
    Matsushita S, Muto M, Suemura M, et al: HLA-linked nonresponsiveness to Cryptomeria Japonica pollen antigen. I. Nonresponsiveness is mediated by antigen-specific suppressor T cell. J Immunol 1987;138:109–115.PubMedGoogle Scholar
  30. 30.
    Eckels DD, Lake P, Lamb JR, et al: SB-restricted presentation of influenza and herpes simplex virus antigens to human T-lymphocyte clones. Nature 1983;301:716–718.PubMedCrossRefGoogle Scholar
  31. 31.
    Austin P, Trowsdale J, Rudd C, et al: Functional expression of HLA-DP genes transfected into mouse fibroblasts. Nature 1985; 313:61–64.PubMedCrossRefGoogle Scholar
  32. 32.
    Natali P, Bigotti A, Cavalieri R, et al: Gene products of the HLA-D region in normal and malignant tissues of nonlymphoid organ. Hum Immunol 1986;15:220–233.PubMedCrossRefGoogle Scholar
  33. 33.
    Ohta Y, Kino I, Kato Y, et al: The distribution and localization of immunoglobulin in the gastric mticosa and gastric cancer. Acta Pathol Jpn 1979;29:523–531.PubMedGoogle Scholar
  34. 34.
    Warren JR, Marshall B: Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1983;i: 1273–1275.Google Scholar
  35. 35.
    Marshall BJ, McGechie OB, Rogers PA, et al: Pyloric campylobacter infection and gastroduodenal disease. Med J Aust1985;142: 439–444.PubMedGoogle Scholar
  36. 36.
    Wyatt JI, Rathbone BJ, Heatley RV: Local immune response to gastric Campylobacter in non-ulcer dyspepsia. J Clin Pathol 1986; 39:863–870.PubMedCrossRefGoogle Scholar
  37. 37.
    Thomas JM, Poynter D, Gooding C, et al: Gastric spiral bacteria. Lancet 1984;ii: 100.Google Scholar
  38. 38.
    Engstrand L, Scheynius A, Påhlson C, et al: Association of Campylobacter pylori with induced expression of class II transplantation antigens on gastric epithelial cells. Infect Immun 1989;57: 827–832.PubMedGoogle Scholar
  39. 39.
    Basham TY, Merigan TC: Recombinant interferon-γ increases HLA-DR synthesis and expression. J Immunol 1983;130:1492–1494.PubMedGoogle Scholar
  40. 40.
    Todd I, Pujol-Borrell R, Hammond LJ, et al: Interferon-y induces HLA-DR expression by thyroid epithelium. Clin exp Immunol 1985;61:265–273.PubMedGoogle Scholar
  41. 41.
    Pujol-Borrell R, Todd I, Doshi M, et al: HLA class II induction in human islet cells by interferon-γ plus tumor necrosis factor or lymphotoxin. Nature 1987;326:304–306.PubMedCrossRefGoogle Scholar
  42. 42.
    Pfizenmaier K, Scheurich P, Schlüter C, et al: Tumor necrosis factor enhances HLA-A, B, C and HLA-DR gene expression in human tumor cells. J Immunol 1987;138:975–980.PubMedGoogle Scholar

Copyright information

© The Japanese Society of Gastroenterology 1992

Authors and Affiliations

  • Nobuaki Ishii
    • 1
  • Mitsuro Chiba
    • 1
  • Masahiro Iizuka
    • 1
  • Hiroyuki Watanabe
    • 1
  • Tomonori Ishioka
    • 1
  • Osamu Masamune
    • 1
  1. 1.First Department of Internal MedicineAkita University School of MedicineAkitaJapan

Personalised recommendations