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Plant Molecular Biology Reporter

, Volume 22, Issue 1, pp 49–52 | Cite as

A protocol for rapid DNA extraction fromArabidopsis thaliana for PCR analysis

  • Ichiro Kasajima
  • Yoko Ide
  • Naoko Ohkama-Ohtsu
  • Hiroaki Hayashi
  • Tadakatsu Yoneyama
  • Toru Fujiwara
Protocols

Abstract

We present a method for instant DNA extraction fromArabidopsis thaliana based on a simple DNA extraction method (Edwards et al., 1991). A piece of rosette leaf (typically 3–5 mg) was ground in a centrifuge tube in extraction solution. Extracted DNA was suitable for PCR analysis, without centrifugation. The feasibility of this method was confirmed by testing 24 primer sets. This method requires less than 1 mg of plant tissue and is useful for genetic mapping, transgene detection, and other experiments.

Key words

Arabidopsis thaliana DNA extraction minute sample PCR analysis 

Abbreviations

EDTA

ethylenediaminetetraacetic acid

WT

wild type

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References

  1. Edwards K, Johnstone C, and Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucl Acid Res 19: 1349.CrossRefGoogle Scholar
  2. Ohkama N, Goto DB, Fujiwara T, and Naito S (2002) Differential tissue-specific response to sulfate and methionine of a soybean seed storage protein promoter region in transgenic Arabidopsis. Plant Cell Physiol 43: 1266–75.PubMedCrossRefGoogle Scholar
  3. Wang H, Qi M, and Cutler AJ (1993) A simple method of preparing plant samples for PCR. Nucl Acid Res 21: 4153–4.CrossRefGoogle Scholar

Copyright information

© Springer 2004

Authors and Affiliations

  • Ichiro Kasajima
    • 1
  • Yoko Ide
    • 2
  • Naoko Ohkama-Ohtsu
    • 1
  • Hiroaki Hayashi
    • 1
  • Tadakatsu Yoneyama
    • 1
  • Toru Fujiwara
    • 2
    • 3
  1. 1.Department of Applied Biological Chemistry, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan
  2. 2.Biotechnology Research CenterThe University of TokyoTokyoJapan
  3. 3.PRESTOJSTJapan

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