Plant Molecular Biology Reporter

, Volume 21, Issue 3, pp 281–288 | Cite as

Efficient validation of single nucleotide polymorphisms in plants by allele-specific PCR, with an example from barley

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Abstract

Although the increasing number of expressed sequence tags (ESTs) from the public domain has facilitated the detection of single nucleotide polymorphisms (SNPs), further validation is needed before they can be used as markers. For SNP validation, we have compared 2 independent methods: (1) the primer extension method followed by capillary electrophoresis on an ABI PRISM 3100 Genetic Analyzer and (2) nested PCR followed by agarose-based visualization. We present an assessment of the efficiency and costs associated with these methods, based on a sample of barley cultivars.

Key words

allele-specific PCR SNP validation 

Abbreviations

AS-PCR

allele-specific PCR

SNP

single nucleotide polymorphism

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Copyright information

© International Society for Plant Molecular Biology 2003

Authors and Affiliations

  • V. D. Soleimani
    • 1
    • 2
  • B. R. Baum
    • 1
  • D. A. Johnson
    • 2
  1. 1.Agriculture and Agri-Food Canada (AAFC) Central Experimental FarmOttawaCanada
  2. 2.Ottawa-Carleton Institute of BiologyUniversity of OttawaOttawaCanada

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