RNA isolation from high-phenolic tea leaves and apical buds
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We describe here a modified procedure based on guanidine hydrochloride to isolate RNA from apical buds of tea containing high levels of polyphenols. The major change was to increase β-mercaptoethanol from 50 to 200 mM in the extraction buffer. This produced phenolic-free and undegraded RNA. The procedure was extended to fully expanded leaves of tea and apical buds of other plant species. The total RNA could be used for mRNA isolation, reverse transcription, and differential display of mRNA. A clean-up protocol to remove chlorophylls that coprecipitate with the RNA also is presented.