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Plant Molecular Biology Reporter

, Volume 19, Issue 2, pp 181–181 | Cite as

RNA isolation from high-phenolic tea leaves and apical buds

  • Lakhvir Lal
  • Rashmita Sahoo
  • Rajesh Kumar Gupta
  • Priti Sharma
  • Sanjay KumarEmail author
Publish by Abstract

Abstract

We describe here a modified procedure based on guanidine hydrochloride to isolate RNA from apical buds of tea containing high levels of polyphenols. The major change was to increase β-mercaptoethanol from 50 to 200 mM in the extraction buffer. This produced phenolic-free and undegraded RNA. The procedure was extended to fully expanded leaves of tea and apical buds of other plant species. The total RNA could be used for mRNA isolation, reverse transcription, and differential display of mRNA. A clean-up protocol to remove chlorophylls that coprecipitate with the RNA also is presented.

Key words

Camellia sinensis Caragana jubata phenolics Potentilla astrisanguinea RNA extraction 

Copyright information

© International Society for Plant Molecular Biology 2001

Authors and Affiliations

  • Lakhvir Lal
    • 1
  • Rashmita Sahoo
    • 1
  • Rajesh Kumar Gupta
    • 1
  • Priti Sharma
    • 2
  • Sanjay Kumar
    • 1
    Email author
  1. 1.Biotechnology DivisionInstitute of Himalyan Bioresource TechnologyPalampurIndia
  2. 2.Division of Agricultural BiotechnologyHimachal Pradesh Krishi VishvavidyalyaPalampur

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