Plant Molecular Biology Reporter

, Volume 19, Issue 2, pp 159–162 | Cite as

Detection of single nucleotide mutations in wheat using single strand conformation polymorphism gels

  • P. Martins-Lopes
  • H. Zhang
  • R. Koebner


We show that single strand conformation polymorphism (SSCP) analysis, using the mutation detection enhancement (MDETM) matrix, is efficient at detecting sequence polymorphisms in PCR amplicons. Four independent wheat genomic fragments were amplified from two contrasting templates and sequenced. The allelic fragments were differentiated at 1–6 single nucleotide positions, but MDE-SSCP was able to unequivocally distinguish each allelic pair. The approach is therefore considered a powerful way of identifying single nucleotide polymorphisms (SNPs) without extensive amplicon sequencing.

Key words

SNP SSCP wheat 



mutation detection enhancement


single nucleotide polymorphism


single strand conformation polymorphism


tris borate ethylene-diaminetetraacetic acid




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Copyright information

© International Society for Plant Molecular Biology 2001

Authors and Affiliations

  • P. Martins-Lopes
    • 1
  • H. Zhang
    • 2
  • R. Koebner
    • 2
  1. 1.Departamento de Gen’etica e BiotecnologiaICETA-Universidade de Tr’as-os-Montes e Alto DouroVila RealPortugal
  2. 2.John Innes CentreNorwich Research ParkNorwichU.K.

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