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Plant Molecular Biology Reporter

, Volume 19, Issue 2, pp 117–123 | Cite as

Karyotype of maize (Zea mays L.) mitotic metaphase chromosomes as revealed by fluorescencein situ hybridization (FISH) with cytogenetic DNA markers

  • Mt Sadder
  • G Weber
Commentary

Abstract

Here we demonstrate fluorescencein situ hybridization (FISH) of chromosome-specific cytogenetic DNA markers for chromosome identification in maize using repetitive and single copy probes. The fluorescently labeled probes, CentC and pZm4–21, were shown to be reliable cytogenetic markers in the maize inbred line KYS for identification of mitotic metaphase chromosomes. The fluorescent strength of CentC signal, relative position, knob presence, size and location were used for the karyotyping. Based on direct visual analysis of chromosome length and position of FISH signals, a metaphase karyotype was constructed for maize inbred line KYS. All chromosomes could be identified unambiguously.

The knob positions in the karyotype agreed well with those derived from traditional cytological analyses except chromosomes 3, 4 and 8. One chromosome with a telomeric knob on the short arm was assigned to 3. A chromosome with a knob in the middle of the long arm was assigned number 4 by simultaneous hybridization with a knob-specific probe pZm4–21 and a chromosome 4-specific probe Cent 4. On chromosome 8, we found an additional small telomeric knob on the short arm. In addition, chromosome-specific probes were employed to identify chromosome 6 (45S rDNA) and chromosome 9 (single-copy probeumc105a cosmid).

Key words

FISH karyotype mitotic metaphase chromosomes Zea mays

Abbreviations

DAPI

4’,6-diamidino-2-phenylindole

FISH

fluorescencein situ hybridization

FITC

fluorescein isothiocyanate

PI

propidium iodide

S:L

approximate arm ratio short:long

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Copyright information

© International Society for Plant Molecular Biology 2001

Authors and Affiliations

  • Mt Sadder
    • 1
  • G Weber
    • 1
  1. 1.Department of Plant Breeding and Biotechnology, Institute of Plant Breeding, Seed Science and Population GeneticsUniversity of HohenheimStuttgartGermany

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